FastQCFastQC Report
Fri 30 Jun 2023
SRR1802139.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1802139.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24902531
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG9773293.9246171403220016No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG8451803.3939522050991524No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA4738541.9028346957985918No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT4238651.7020960640506781No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA3473671.3949064052967146No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT3232341.2979965771350712No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA2656001.066558254660942No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG2479310.9956056273958659No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG2287590.9186174690436085No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1770550.7109919871197028No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1515090.6084080369180145No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1510300.6064845376560318No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1300460.5222200104880905No Hit
CACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATC1277760.5131044711880893No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG1274230.5116869445920978No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG1265960.5083659970145203No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1239480.4977325397165453No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1094100.43935293163574424No Hit
AAACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAA1078240.43298410109398117No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAGA1041020.4180378291668425No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC968030.38872755544406307No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG962270.38641453754238875No Hit
GGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATCG940290.3775881254800968No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG876050.3517915508267011No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG837280.3362228522072716No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT826640.331950194138901No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAGA813770.3267820447648474No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG752450.30215804168660604No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG751510.3017805700151523No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATA740290.2972750038941825No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC730530.29335572356078987No Hit
AGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCTGTAGGCACCATCAAT707780.28422010598039216No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATC696040.27950572574329896No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG678290.2723779362025491No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAGAT672700.27013318445422274No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA661220.2655232112751913No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA657430.2640012776211382No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATAG644370.258756830781578No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT627490.2519784033197268No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA598570.24036512593840362No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC565220.2269729129139524No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC557840.22400935872743216No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA555590.22310583610959064No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA541680.21752005850329026No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA533990.21443201897831188No Hit
CGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGAT514940.20678219414725355No Hit
ACCTCTAGCATCACCAGTATTAGAGGCTGTAGGCACCATCAATAGATCGG504740.20268622494637192No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA495970.19916449456482957No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGAT494830.1987067097717899No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA474620.19059106883553323No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCCTGTAGGCACCATCAATAGATC461610.18536670027636948No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATAGATCG460910.1850856043508188No Hit
ACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATAGATC457220.18360382725755867No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATAG447860.1798451731673379No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA444770.17860433543883553No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG439380.17643989681209513No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAA439150.17634753672227133No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT430840.17301052652037657No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG428850.17221141096059675No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG418560.16807930085500145No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCCTGTAGGCACCATCAAT414450.16642886620641092No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG414080.16628028693147695No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATAGA413740.16614375462478093No Hit
GAAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAG412540.16566187589526543No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGCCTGTAGGCACCATCAATA405610.16287902623231348No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGCTGTAGGCACCATCAATAGAT402110.16147354660455998No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATCG389760.1565142113466298No Hit
CAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCGG381140.15305271580627688No Hit
ATGCAGCTCAAAACGCTTAGCCTAGCCACACCTGTAGGCACCATCAATAG379460.1523780855849552No Hit
AGTGCGGTAACGCGACCGATCCCGGAGACTGTAGGCACCATCAATAGATC376350.15112921654429423No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGCTGTAGGCACCATCAATAGATC368040.1477922063423995No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA359200.1442423663683021No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAGA357430.14353159524226675No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG356700.14323845234847815No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAATA348820.14007411535799313No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA346920.13931114070292694No Hit
AGAAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATA334760.13442810291050336No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT333310.13384583277900547No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATCG328090.1317496603056131No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGAT327360.13145651741182451No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGATC326960.1312958911686527No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG324200.13018757009076706No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGGGCTGTAGGCACCATCAATAGAT321280.1290149985156127No Hit
AATGCAGCTCAAAACGCTTAGCCTAGCCACACCTGTAGGCACCATCAATA319920.1284688692888285No Hit
AGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGG308070.12371031683486308No Hit
TGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCGG301660.12113628128803454No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA292040.11727322013975205No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCCTGTAGGCACCATCAATAGATCGG288240.11574727082961969No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG286820.1151770476663597No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCACTGTAGGCACCATCAATAGATC285360.11459076187878253No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGAT282390.1133981120232317No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTACTGTAGGCACCATCAAT278180.1117075208138482No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT275750.11073171638657933No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG274960.11041447955631498No Hit
CACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATAGAT274520.11023779068882597No Hit
CCCCTTGTAAATTTAACTGTTAGTCCACTGTAGGCACCATCAATAGATCG272240.10932222110274654No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT271190.10890057721442051No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCAACTGTAGGCACCATCAATAGAT264420.1061819780487373No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGATC262850.10555152004428786No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATCA262060.10523428321402352No Hit
AAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGA261360.1049531872884728No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA260000.1044070580616886No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGCTGTAGGCACCATCA254840.102334979524772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)