FastQCFastQC Report
Sat 1 Jul 2023
SRR1802141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1802141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28537500
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA17763526.2246237406920715No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT12440744.359435830048182No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG11335193.972033289531318No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG8063322.8255173017958826No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG7668642.6872150678931233No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT3217931.1276145422689443No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA2944371.031754708716601No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA2935061.0284923346473938No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA2767980.9699448094612352No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT2509440.8793482260183968No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT2158850.7564958388085852No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG2133270.7475321944809462No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG2105940.7379553219448095No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG2095330.7342374069207184No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG1749400.613017958826106No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1523840.5339780989925537No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1424170.49905212439772234No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG1363920.4779395532194481No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA1334040.46746911957950066No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA1332990.4671011826544021No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA1307400.45813403416557164No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG1203220.4216276828734122No Hit
GCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCTGTAGGCACCA1177070.41246430135786244No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1128460.3954305738063951No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC1108940.3885904511607534No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1087280.38100043802014893No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1066080.37357161629434954No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1033000.36197985107314934No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA974910.3416241787122208No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG937970.3286798072711345No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC921230.3228138414367061No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG908920.31850021901007447No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA889480.3116881296539641No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATC888390.3113061760840999No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGA872400.3057030223390276No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC865410.3032536136662286No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA861670.30194305738063953No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGATC819480.2871590013140604No Hit
AAACCTGCATTAAAAATTTCGGTTGGGCTGTAGGCACCATCAATAGATCG794640.2784546649145861No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA776190.27198948751642577No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATAGATCG773630.27109242225142355No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA760550.26650897941305296No Hit
CGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCTGTAGGCACCAT733630.2570757774857644No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC690680.24202540516863774No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT668040.23409198423127464No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA645660.22624967148488828No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATCA632270.2215575996495839No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA616770.2161261498028909No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC609000.21340341655716163No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATA599910.21021813403416556No Hit
GGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATCG583070.20431712658782303No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC565110.19802365308804207No Hit
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCAT557720.19543407796758652No Hit
ACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCA537270.18826806833114323No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAA513090.17979500657030223No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC488930.17132895313184407No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG463440.16239684625492773No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT461970.16188173455978977No Hit
CACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATC448130.15703197547087167No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAGA439800.15411300919842313No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGAT423200.14829610162067455No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA420780.14744809461235217No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAGA416680.1460113885238721No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA416040.14578712220762155No Hit
AAACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAA405270.1420131406044678No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCA398060.13948664038545774No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG391010.1370162067455103No Hit
AGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCTGTAGGCACCATCAAT390230.13674288217257993No Hit
ACCTCTAGCATCACCAGTATTAGAGGCTGTAGGCACCATCAATAGATCGG384750.1348226018396846No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAATAG373980.13104862023653088No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAGA372840.1306491458607096No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATAG372130.13040035041611914No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAG356730.12500394218134034No Hit
TGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCGG356390.12488480070083224No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCTGTAGGCACCATCAATA354380.12418046430135786No Hit
CCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGCTGTAGGCACCAT336050.11775733683749452No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA335570.11758913710030662No Hit
ACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATAGATC331200.11605781865965835No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGCTGTAGGCACCATCAA329440.11544108628996934No Hit
GAAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAG327900.11490144546649145No Hit
CCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCCTGTAGGCACCATC327180.1146491458607096No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG326120.11427770477441962No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA324210.1136084099868594No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCCTGTAGGCACCATCAATAGATCGG318730.11168812965396409No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG316090.11076303109943057No Hit
TGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGCTGTAGGCACCATCAA314840.11032501095050373No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG313360.10980639509417434No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCACC309230.10835917652212003No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT308850.10822601839684624No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG307520.10775996495838809No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG306410.10737100306614103No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA299750.10503723171265877No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCAT297800.1043539202803329No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGGCTGTAGGCACCATCAATAG294120.10306438896189225No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATCG291010.10197459483136223No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATAGAT289170.10132982917214191No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)