FastQCFastQC Report
Fri 30 Jun 2023
SRR1802153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1802153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18400100
Total Bases938.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2670441.4513181993576123No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA1940761.0547551372003412No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG1918101.0424399867392025No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1855771.008565170841463No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1837360.9985597904359215No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC1221730.6639800870647442No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGAT1061950.5771436024804213No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1050700.5710295052744279No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1038860.5645947576371868No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA1033140.5614860788800061No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA900020.4891386459856197No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA857040.4657800772821886No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG852880.4635192200042391No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC829920.45104102695094045No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG758130.41202493464709433No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG687690.37374253400796736No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA663150.36040564996929364No Hit
GGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATCG637780.3466176814256444No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT631850.34339487285395187No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG622340.33822642268248543No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT618170.33596013065146385No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA616910.3352753517643926No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA599570.3258514899375547No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA595740.3237699795109809No Hit
TCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAG582950.31681893033190034No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG581190.3158624137912294No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG576150.3131232982429443No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT569070.3092754930679725No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT509000.27662893136450345No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG489160.26584638126966703No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA486640.2644768234955245No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA457110.2484279976739257No Hit
GCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT438540.23833566121923253No Hit
TGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA437610.23783022918353708No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGAT434840.23632480258259464No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT432830.23523241721512383No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA419940.22822702050532334No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATA418380.22737919902609227No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG410430.2230585703338569No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATAGATC405450.2203520633040038No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAAT381540.2073575687088657No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC381340.20724887364742584No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA362550.19703697262514877No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC346170.1881348470932223No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT344310.1871239830218314No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC344020.18696637518274356No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGCTGTAGGCACCATCAATAGA336740.1830098749463318No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA335370.18226531377546862No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAG334720.18191205482578898No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG330860.17981424013999925No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC329630.17914576551214395No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG327540.17800990212009718No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC316520.1720208042347596No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC312300.16972733843837803No Hit
CGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA309620.1682708246150836No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA304530.16550453530143858No Hit
TAAACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCA297810.1618523812370585No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCA288960.15704262476834366No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGGCTGTAGGCACCATCAATAGAT286850.15589589187015288No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC286410.15565676273498513No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC283080.15384698996201107No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC282310.15342851397546753No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA280480.1524339541632926No Hit
AAAACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCA277580.15085787577241427No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATAG270750.14714593942424226No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG267840.14556442628029195No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA264920.14397747838326966No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA263120.1429992228303107No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGCTGTAGGCACCATCAATAGA261310.14201553252427976No Hit
TTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAA260270.14145031820479237No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA259130.14083075635458503No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAGA255350.13877641969337123No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA255070.1386242466073554No Hit
CCGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGAT252900.13744490519073266No Hit
CGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATAGATCG249190.13542861180102284No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT248520.13506448334519922No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCG237930.1293090798419574No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA237490.12906995070678964No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG234200.12728191694610355No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA226540.12311889609295601No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG222730.12104825517252624No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATA220510.11984173999054354No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG220030.11958087184308781No Hit
TCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCTGTAGGCACCA216470.11764609974945787No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG213640.11610806463008352No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA211920.11517328710170054No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC208190.1131461242058467No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATA206330.11213526013445578No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT204240.11099939674240901No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACTGTAGGCACCATCAA203650.11067874631116134No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT202690.1101570100162499No Hit
TTGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGAT202690.1101570100162499No Hit
CGCGCGCTACACTGACTGGCTCAGCGTGCTGTAGGCACCATCAATAGATC198010.10761354557855664No Hit
CCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC193870.10536355780675105No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC192610.10467877891967979No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA189960.10323856935560133No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAATA189740.10311900478801746No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA189530.10300487497350558No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT188510.10245053016016217No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA187300.10179292503845087No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)