FastQCFastQC Report
Fri 30 Jun 2023
SRR1802154.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1802154.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14533146
Total Bases741.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA2948122.0285490835913986No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT2020781.3904628770673604No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1921761.3223289713046302No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG1515361.0426923392911625No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1459661.0043661571967968No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1382660.9513838228832215No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1190430.8191137693105126No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1039590.71532344063701No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC884550.6086431664554942No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA822290.5658031647105176No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA797640.5488419369075354No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG783030.5387890550332324No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA749440.5156763717917648No Hit
TCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAG734900.5056716556759286No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGAT673840.4636573526475273No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA646440.44480389861906017No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC618080.425289885617333No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC574270.39514500163970007No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA517600.3561513797494362No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA500560.34442645797406835No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG491440.33815114772809685No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA474240.326316132790519No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG464410.3195522841372405No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT438220.30153140964798675No Hit
GGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATCG435940.29996258208649385No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG431000.29656345570325926No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA424980.29242120047510706No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA416630.2866757135722713No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG400330.2754599726721248No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA383620.26396211804381514No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA376130.25880838188785826No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG370360.25483814722565923No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA363030.24979450423191235No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG353600.2433058884841589No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA351420.24180586914904728No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT351100.24158568282462725No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG351040.24154439788879847No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG347580.23916363325600667No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGAT346870.23867509484869964No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT335010.23051443919988143No Hit
TTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAA328680.22615887846994726No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT327940.2256496975947259No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAAT324460.2232551713166578No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG308860.2125210880011802No Hit
TGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA297280.20455309538622954No Hit
GCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT291560.20061726483722106No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA289550.1992342194869576No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA283310.19494058616076657No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA280970.1933304736634449No Hit
TAAACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCA275750.1897386842463428No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATA269740.18560330984082868No Hit
AAAACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCA263590.18137160391838078No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC249640.17177285633819409No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG242880.16712142023482046No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG237260.1632543979121933No Hit
CGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA237220.16322687462164076No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA236930.1630273307651351No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG236080.1624424608408943No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAG225090.1548804367615931No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA224030.15415106956195168No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC221390.15233453238548625No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC217720.14980927047729375No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGCTGTAGGCACCATCAATAGA216940.1492725663115199No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAAT215990.14861888816089786No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA215520.1482954894969059No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC211390.1454537097473596No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC210880.14510278779281513No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATAGATC207880.14303854100137714No Hit
TCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATAGATCGGAAGAGC207050.1424674327224126No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGGCTGTAGGCACCATCAATAGAT207020.14244679025449825No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC206330.1419720134924675No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT200510.1379673747170778No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCG199180.13705222530620692No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT196790.13540770869569466No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATAG195560.13456136751120507No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGCTGTAGGCACCATCAATAGA194810.1340453058133456No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT194780.1340246633454312No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG193460.1331163947571985No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAGA192590.13251776318768146No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG184670.12706815165828514No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCA182260.12540987340249662No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA181530.12490757334991337No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT179280.12335938825633486No Hit
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATC178580.122877730671666No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC174940.12037311123138789No Hit
CGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATAGATCG174590.12013228243905347No Hit
TCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCTGTAGGCACCA174540.12009787832586283No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAG170540.11734554927061217No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC168950.11625149847115002No Hit
CCGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGAT168560.11598314638826307No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATA167040.11493726134726784No Hit
ACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATAGATC159500.10974912107812032No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT155950.10730642904158535No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA154400.1062399015326757No Hit
TTGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGAT151160.10401051499792269No Hit
TTGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC150980.10388666019043641No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA147680.1016159887198546No Hit
CCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC146310.10067331601843126No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT145760.1002948707733343No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)