FastQCFastQC Report
Fri 30 Jun 2023
SRR1802155.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1802155.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19514203
Total Bases995.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT6718073.442656612724589No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA2910651.4915546384343752No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1778330.9113003487767345No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1747600.8955528442540032No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1728140.88558062043323No Hit
CGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATAGATCGGAAGAGC1697110.8696793817303223No Hit
TCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1580470.8099075324777547No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1536450.7873496037732108No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1476500.7566283901013021No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG1381380.707884406040052No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG1279410.6556301581981083No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA1275280.6535137509843472No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC1232120.631396526929642No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC1122210.5750734477856974No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT966120.4950855538399391No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGAT884060.45303413108903295No Hit
CGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAATAGATCGGAAGAG881390.4516658968854633No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA877130.4494828715269591No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG818670.4195252042832598No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG775410.3973567355018291No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC758080.3884760243603082No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATAGATC743560.38103529003977255No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA735200.37675123088552476No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG717520.3676911632004648No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA700280.35885657231299684No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG667960.3422942766353307No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG666250.34141799180832544No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA661520.33899411623421155No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCG638670.3272846961774457No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA625070.32031541334278424No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG617960.3166719132726046No Hit
GGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATCG607750.3114398266739359No Hit
TCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATAGATCGGAAGAG582580.2985415289571396No Hit
CGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATAGATCG566510.2903065013723594No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT533330.27330350104485435No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA465710.23865181683310355No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG463780.23766279360730233No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA457270.2343267618974754No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT456550.23395779986505214No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATAGA444340.22770081873187442No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCA438680.22480036719921384No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC438100.22450314778420621No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA435670.2232579009247777No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATAG425830.2182154198149932No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG413310.2117995800289666No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC411890.21107190490946515No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG411880.2110667804367926No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT405730.2079152297431773No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG402380.20619853139787467No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT398960.2044459617438642No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA394070.20194009460698958No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA389920.19981343844788332No Hit
TGCACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGA388970.19932661354399153No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA363360.1862028390296032No Hit
CGCGCGCTACACTGACTGGCTCAGCGTGCTGTAGGCACCATCAATAGATC356750.18281556259305082No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCA353530.18116548239249125No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT351450.18009959207660184No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA345670.17713764687187072No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT345210.17690192112893363No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA341990.17525184092837406No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC341110.17480088733319007No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT332490.1703835918894561No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC332120.17019398640057193No Hit
TCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAATAGATCGGAAGA325910.16701168887092135No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC323490.1657715664841654No Hit
TTGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC317730.16281987022477934No Hit
TGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA315830.16184622041699576No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGA313680.16074445879239854No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG311630.15969394189452676No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG311030.15938647353417407No Hit
CCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC307340.15749554311800487No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTGCTGTAGGCACCATCAATAGAT300750.15411851562679757No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA296690.1520379797217442No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC296410.15189449448691295No Hit
TCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC292910.15010092905152211No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA288980.14808701129121185No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT285580.14634469058254648No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC284780.14593473276874286No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT283030.14503795005104744No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGAT277890.1424039710973592No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGCTGTAGGCACCATCAATAGA276120.14149693943431868No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC275750.14130733394543452No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAAT275240.14104598583913472No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGATC274110.1404669204271371No Hit
CGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA272330.13955476429142405No Hit
CGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCA267340.13699765242782397No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATC265350.13597788236598748No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGCTGTAGGCACCATCAATAGA264690.1356396671695995No Hit
GCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT262740.13464039499845318No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA260020.13324653843152087No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGGCTGTAGGCACCATCAATAGAT258780.13261110382012528No Hit
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG254400.13036658478955046Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGAT252100.12918795607486505No Hit
TCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAG247850.12701005518903336No Hit
TAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG242110.12406860787499238No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC237380.1216447323008785No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCA233010.11940533774297624No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAGA228590.11714032082171125No Hit
ACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCA225550.1155824811292575No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAACTGTAGGCACCATCAATA223900.11473694313828753No Hit
TGCACGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATAG219400.11243093043564217No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC214520.10993018777144012No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAG204860.10497994716976143No Hit
GCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC203470.10426764546827764No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATAG203330.104195902850862No Hit
TTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG201320.10316588384368042No Hit
TTGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGAT198200.10156704836984633No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAATAGA196850.10087524455905271No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGTAGGCACCATCA196030.100455037799904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)