FastQCFastQC Report
Fri 30 Jun 2023
SRR1803148.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803148.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151278196
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA93538596.18321691250205No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC71759114.743519680787309No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG41570412.747944588128219No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC25860761.709483632393395No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT21331051.4100544932463366No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG19807641.309351943884894No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC18229541.2050342006986914No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT13667940.9034970247794335No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG12183240.8053533372383684No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG10934980.7228391327458717No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC10564500.6983491527093567No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCAC8850640.5850572147224706No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG7789790.5149314445817427No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT7703050.5091976374440637No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC6803510.44973500345019984No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG6440220.4257203067122773Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCA5771890.3815414350922059No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA5681760.3755835374980278Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGAGATCGGAAG5290330.3497086916610243No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG5237880.3462415694063406No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGATCGGAAGAGCACA4769140.31525627130032674No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCAGATCGGAAGAGCAC4762140.3147935476438389No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG4676320.30912055561529833No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT4335410.28658525251054684No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC4268180.2821411223068789Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG4243580.28051497917122176TruSeq Adapter, Index 1 (100% over 49bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG4065590.26874923865432665No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA4040630.2670992983020501Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGACTCTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAGAGCACAC4017070.26554190268107114No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA3987740.26360309056038717No Hit
AGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGT3762500.2487139653621993No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA3664700.24224905484726958No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAAAGATCGGAAGAGCACACGTCT3600500.2380052178834814Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG3553060.23486927356008397Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCACA3518530.2325867238660091No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCAC3449300.22801038690334463No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC3355360.22180063543327816No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTC3178070.21008116728203183Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG3096870.20471357286677322Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG3054450.20190946750845706No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATACAGATCGGAAGAGCA3025090.19996867228638818No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT2968320.196215983432272Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2893270.19125492480092768Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTAATGATGAGATATAACCTTGACTGAAGAGATCGGAAGAGCACACGTC2807830.18560705205659644No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT2765770.18282674391489967No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC2712640.17931467136215717No Hit
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCGGAAGAGCACACGTC2706860.17893259382865723No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2695040.17815125188298783No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACAC2650730.17522221113741998No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG2634400.1741427429502134No Hit
ACGAATTTTTGAGCGGGTAAAGGTCGCCCTCAAGAGATCGGAAGAGCACA2521160.16665719625583056No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT2488880.16452337916562676Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC2462350.16276965650753794No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC2457390.16244178374522658No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC2419890.15996290701404187No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC2360890.15606280762364458No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2358990.1559372112025979No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG2317800.15321441300106461Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA2306290.15245356310303965Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG2278880.15064166947099236No Hit
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA2263770.1496428474067737Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2262680.14957079472312057Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA2221340.14683808101466256No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC2181140.14418072515883254Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGAGCACACGTCT2172440.14360562575719768Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT2126690.14058139614515233Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA2097480.1386505164300082TruSeq Adapter, Index 1 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG2059740.1361557748877439No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACAC2053960.13577369735424397No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGGAAGAGCACACGTCT2041130.1349255909952813Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTAGAGATCGGAAGAGCACACG2004320.1324923255959504No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT1924020.12718422422224018No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG1888370.12482763874312726Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGAAACTCGACTGCATAATTTGTGGTAGTGGAGATCGGAAGAGCACAC1846660.12207046678425489No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA1803770.11923529283757457Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1776990.11746504433461118No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT1743670.11526247972972918Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACAC1739940.11501591412420067No Hit
GAGACTCTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAGAGCACA1733220.11457169941397238No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC1720260.11371499961567497No Hit
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1693910.11197317556589582Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGTCT1688760.11163274316147981Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC1643810.10866139625303306No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTAGATCGGAAGAGCACAC1630910.10780866265750551No Hit
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGA1626790.1075363167339727Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGT1624520.10738626206251164No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG1618640.10699757419106187No Hit
AGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCAGATCGGAAGAGCACAC1609860.10641718651906715No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT1599180.10571120242602577No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGAGATCGGAAGAGCAC1594930.10543026306315814No Hit
CTGGGTCGGGGTTTCGTACGTAAGATCGGAAGAGCACACGTCTGAACTCC1588300.10499199765708471Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAA1545280.10214823027106959No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCCAGATCGGAAGAGCACAC1515140.10015587441299208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)