FastQCFastQC Report
Fri 30 Jun 2023
SRR1803149.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803149.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80965178
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG1258930415.549035166698456No Hit
GCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTG27849393.439675016832545Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGA27525683.399693631254661Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT22421132.769231236668188No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC20278182.5045557239434464No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT16575422.047228254102029TruSeq Adapter, Index 1 (100% over 35bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCA13472841.6640289483461645No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA12583881.5542335990418004No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG12004481.482671970411774No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAC10035201.2394464197929633No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA9743371.2034025294182642No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC8660951.0697129573407471No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGC7786950.9617653159485426No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG6972230.8611393406681574No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCG6730270.831254888366947No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAA6121660.7560855359324968Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG5701630.7042076780217786No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGAT4234610.5230162033362046No Hit
GTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAG3588990.4432757499773544No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT3293970.40683786306256253No Hit
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTC2862490.35354581694367426No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGA2804360.34636618720210804TruSeq Adapter, Index 1 (100% over 36bp)
GCGCGACCGCTCTCCAGATCGGAAGAGCACACGTCT2725170.3365854392366061Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC2634390.32537321167872935Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACCGCTCTCAGATCGGAAGAGCACACGTCTGAAC2471180.3052151629926633Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGC2436350.3009133136223081No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACA2378190.29372997858412664No Hit
CGACCGCTCTAGATCGGAAGAGCACACGTCTGAACT2284550.2821645127489252Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGCGCGACCGCTCTCAGATCGGAAGAGCACACGT2271420.2805428279302986No Hit
GCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAA2215930.27368926429087825Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGA2205590.2724121720574739No Hit
CGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCT2182520.26956279896031354Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC2177720.26896995150186664No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA2109530.26054781229530555TruSeq Adapter, Index 3 (100% over 36bp)
CGGCGCGACCGCTCTCAGATCGGAAGAGCACACGTC2055210.25383875522388155No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGA1829810.22599962665431303No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC1722740.2127754230343321No Hit
CTGGGTCGGGGTTTCGTACGTAAGATCGGAAGAGCA1573120.19429587371499388No Hit
CGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGA1567850.19364497660957405Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAA1554670.19201711629658866No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATC1546470.19100433522174187No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC1463520.18075919008045657No Hit
CGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAA1440710.17794192955396207Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATA1430720.17670806578106948No Hit
TCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGG1372430.16950867445755508No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAG1361240.16812659882005077No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCG1298350.16035906201552474No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1285770.15880530763484518No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTG1266440.1564178615157247Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGATCGG1262320.15590900078055778No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGG1254270.15491474618878748No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCAGAT1244320.15368582281138196No Hit
CGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGA1156530.1428428898161627No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGG1142770.14114339376861496No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAGA1088230.13440716452201218No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGA1070730.13224574149642454No Hit
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAAC1047710.12940254389362302Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC984220.12156090115679115No Hit
TTTCGTACGTAGCAGAGCAGCAGATCGGAAGAGCAC969920.11979470977016811No Hit
TTTCGTACGTAGCAGAGCAGATCGGAAGAGCACACG968710.11964526280668461No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCAC952180.11760364437165814No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG929380.11478761894403541Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA926020.1143726257231226Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT919500.11356734125873225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG918070.11339072212006994No Hit
GGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAG907540.11209016300810207No Hit
GGCGCGACCGCTCTCAGATCGGAAGAGCACACGTCT896990.11078713369839067Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)