FastQCFastQC Report
Fri 30 Jun 2023
SRR1803150.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803150.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98011215
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG80785708.242495514416385No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT33988283.467794986522716No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG27938202.8505105257597307No Hit
GCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTG27722572.8285099822505004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGA22654262.311394670497657Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCA19296661.968821629239062No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT18469641.8844414896805433TruSeq Adapter, Index 1 (100% over 35bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC14984721.5288780982870174No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAA13344021.3614788879007367Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA12943261.3205896896594946No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC11607751.1843287525820387No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCG10681721.0898467078486886No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGC10253551.0461608908735598No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG9404420.9595248870244084No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAC6462970.6594112724752978No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA6083580.6207024369609131No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG6007470.6129369990975012No Hit
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAAC4800200.48976027896399404Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG4387250.44762734550326716No Hit
GCGCGACCGCTCTCCAGATCGGAAGAGCACACGTCT4373340.44620812016257533Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAG4182150.42670116884072906No Hit
GTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAG3655690.3729869076717394No Hit
GCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAA3355910.3424006120115948Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC3341140.3408936416102994No Hit
CGACCGCTCTCAGATCGGAAGAGCACACGTCTGAAC3307620.3374736248295667Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC3263580.33298026149354437Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGC3218000.3283297732815576No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACA3150420.32143464398436444No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC2718510.27736723802475055No Hit
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTC2711780.27668058191095785No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT2664310.27183725862392377No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTG2654550.27084145421521405Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGA2648950.27027009102988875No Hit
CTGGGTCGGGGTTTCGTACGTAAGATCGGAAGAGCA2596330.26490131767063596No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAC2516230.2567287835376798No Hit
ACGGCGCGACCGCTCTCAGATCGGAAGAGCACACGT2510170.2561104869478457No Hit
CGACCGCTCTAGATCGGAAGAGCACACGTCTGAACT2498910.254961638828781Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG2299160.234581318066509Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGCGACCGCTCTCAGATCGGAAGAGCACACGTC2297900.23445276134981086No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT2266810.2312806753798532Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA2263070.2308990863953681Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATC2216370.2261343255463163No Hit
TTTCGTACGTAGCAGAGCAAGATCGGAAGAGCACAC2174100.22182155378851287No Hit
TTTCGTACGTAGCAGAGCAGATCGGAAGAGCACACG2161590.22054516924415232No Hit
CGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGA2160490.220432937189892Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCT2045000.20864959178396061Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA2043130.2084587972917181TruSeq Adapter, Index 3 (100% over 36bp)
TTTCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAG2037950.20793028634529223No Hit
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAA1961730.2001536252764543No Hit
TTTCGTACGTAGCAGAGCAGCAGATCGGAAGAGCAC1944000.19834464862005843No Hit
GCAGAGCAGCTCCAGATCGGAAGAGCACACGTCTGA1936360.19756514598865035Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGG1797250.18337187229032922No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGAT1687290.17215274802990657No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCG1684680.1718864519738889No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAGA1634380.16675438621998512No Hit
CGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAA1605950.16385369776305703Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTTCGTACGTAGCAGAGCAAGATCGGAAGAGCACA1578310.16103361232691585No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGA1575290.16072548432340114No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGA1574750.16067038858767335TruSeq Adapter, Index 1 (100% over 36bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAAGATCGG1557770.15893793378645493No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT1535640.15668002891301777Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATA1515390.1546139388232255No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1481060.1511112784389011Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG1455200.14847280487238118No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT1419010.14478037028721663No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG1414840.14435490877242976No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCAC1401680.14301220528691538No Hit
GTTTCGTACGTAGCAGAGCAGATCGGAAGAGCACAC1389170.1417358207425548No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT1357560.13851067961967414No Hit
TCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTC1323120.1349967960299237No Hit
TCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCA1310550.13371428973714897No Hit
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAAC1288830.1314982168112088Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC1280290.13062688795358776No Hit
GAGATGGAATAGATCGGAAGAGCACACGTCTGAACT1237860.12629779153334647Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCGTACGTAGCAGAAGATCGGAAGAGCACACGTC1231180.12561623687656562No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC1226900.1251795521563527No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGAT1222780.12475919209857769No Hit
GTTTCGTACGTAGCAGAGCAGCAGATCGGAAGAGCA1217590.12422966086074946No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGA1191390.12155649738654908No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAT1171140.1194904072967568No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCAGAT1164310.11879354826894045No Hit
CGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGA1159180.1182701387795264No Hit
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAA1121070.11438180824510746Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCGCGACCGCTCTCAGATCGGAAGAGCACACGTCT1120020.11427467764785898Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATC1116230.11388798720636205No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAAGATCG1102740.11251161410456957No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGATCGG1096090.1118331203219958No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA1083110.11050878208172402No Hit
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC1044490.10656841668578439Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT1009560.10300453881731801No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGA1008570.1029035299684837No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)