FastQCFastQC Report
Thu 6 Jul 2023
SRR1803153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166536142
Total Bases8.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA145230908.720683585908938No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG70964794.261224569499154No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC70033924.205328594678265No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC34166792.0516141174928864No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT33895332.0353137518941686No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC31837171.9117273654628075No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC19048921.1438309889513352No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT17050681.023842620300403No Hit
CTGGGTCGGGGTTTTGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA14420890.865931552563527No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT13950840.8377064481294396No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG12268090.7366623156191525No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC11986850.7197746901090094Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG11683470.7015576234496894No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGATCGGAAGAGCACA9109670.5470085886822093No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG7792280.4679032374846296Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACAC7667810.4604291841947437No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCAC7111820.42704363837130316No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG6830310.41013980016421897No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG6469050.3884472116569147Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC6467080.38832891901626976No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT6249270.37525007634679086No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA6006340.36066285239152474Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTC6004930.3605781860852763Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGT5766930.3462869939667511No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG5602140.33639184460031507No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA5515480.33118816935245204Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG5469950.32845422827196274No Hit
ATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGAGCACACGTCT5193670.31186443600933184Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC4326450.2597904543747627No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT4128310.2478927367009619No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGTCT4092690.24575386164523974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG3910890.23483731237150912No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC3901340.23426386327599688No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCAC3753890.2254099293353391No Hit
TTTTGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTC3667700.22023447618955888Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG3588160.21545833576473747Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC3587970.21544692682985297No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGT3576050.21473116628341252No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT3534320.21222540390061395Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3385560.20329280835627858TruSeq Adapter, Index 1 (100% over 49bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC3217410.1931959009834634No Hit
AGACTCTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAGAGCACAC3176380.1907321715186605No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCA3125190.1876583642726634No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC3095800.18589358218710267No Hit
AAGCTACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGT3093740.18576988531414398No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT3090460.18557293106982148Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGATCGGAAGAGCACACG2989820.17952979840255937No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACAC2935440.17626444114455347No Hit
ATTAATGATGAGATATAACCTTGACTGAAGAGATCGGAAGAGCACACGTC2932610.17609450806179958No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT2744320.16478825359122345Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA2739400.16449282222473965Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2687640.16138478817408897Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2577400.1547652040600292Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAAGATCGGAAGAGCACACGTCT2544570.1527938602060326Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG2512170.15084833657309055No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGATAGATCGGAAGAGCACACGT2482120.14904392345056247No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA2472840.1484866870519914No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC2433250.14610942530420815TruSeq Adapter, Index 1 (100% over 36bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA2405630.14445092645415072No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA2405260.14442870905463873Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG2404250.14436806155867357No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAGATCGGAAGAGCACAC2404020.1443542507427607No Hit
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTC2395820.14386186513195437Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGAGATCGGAAGAGCACAC2319230.1392628634329718No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2268970.13624489992088323Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGGTCGGGGTTTCGTACGTAAGATCGGAAGAGCACACGTCTGAACTCC2241150.1345743916656842Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG2237910.13437983930238998No Hit
ATTGATCATCGACACTTCGAACGCACTTGAGATCGGAAGAGCACACGTCT2172110.13042874501079774Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAGAGCAC2170470.13033026788863644No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC2153430.12930706657057062No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2150250.12911611702881887No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC2147350.12894198065426543No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2136440.12828686760379018No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACACG2079030.12483956785788877No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2028910.12183001092939934Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT2008620.12061165677778221Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1987040.11931584196300164No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGGAAGAGCACACGTCT1918780.11521703198816746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCT1906530.11448145592324337Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1893580.11370384694032362Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTTGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC1869100.11223389575098959Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCT1864030.11192945733064959Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGA1858230.11158118458154266Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTCT1850940.11114344176413068Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCA1844330.11074653092420023Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG1842600.11064264956972523Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1815910.10903999445357633No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1800560.10811827260895716TruSeq Adapter, Index 1 (100% over 35bp)
AGTTCAAGAGGGCGTGAAACCGTTAAGAGGAGATCGGAAGAGCACACGTC1792900.1076583123920332No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC1766860.10609468784259456No Hit
GTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACT1748720.10500543479624981Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACAC1736660.10428126766621026No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGAGATCGGAAGAGCAC1727450.10372823455943876No Hit
AAGAAATTCAATGAAGCGCGGGTAAACGGAGATCGGAAGAGCACACGTCT1714380.10294341993343403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1694420.10174488130030056TruSeq Adapter, Index 1 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)