FastQCFastQC Report
Fri 30 Jun 2023
SRR1803154.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803154.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142686297
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAN47499943.3289769934950377No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAN40165182.814929032743768No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCN26764971.8757911980854056No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAN21458651.5039040504359014No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATN19635161.376106915158083No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAN19252801.3493096677671856No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGN14471741.0142347446300328No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAN14250260.9987125813489995No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATN13763050.9645670459862029No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACN12031530.8432155191468736No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAN9632230.6750634225233275No Hit
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAN9571010.6707728913870405No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCN8805820.6171454572123348No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN8699760.6097123678246412Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGN8271290.5796835557376614No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACN8235840.5771990845063418No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATN7833930.5490316985379472No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGAN7462960.5230327057965489No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCAN7410370.5193469979811727No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCN7008740.4911992354808955No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAN6972160.48863556953895865Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCN6829400.4786304041515634No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCN6593790.46211795656873766No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCN6233900.4368954924942793No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCN5890040.4127964719695543No Hit
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACN5765400.40406122530462757No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTN5671440.3974761500748737No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGN5651000.39604363690228783No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATN5617480.3936944274333505No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN5573860.390637371435885Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAN5390700.37780081993437675No Hit
GGGATGCGTGCATTTATCAGATCAAAACCAACAGAN5225720.36623839218421933No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAN5176000.36275382491704866No Hit
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACN4726820.3312735770275123No Hit
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCAN4492650.3148620501378629No Hit
ATTTATCAGATCAAAACCAACCAGATCGGAAGAGCN4449180.3118155067126032No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCN4382470.30714021543358155No Hit
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACAN4251520.2979627398978614No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGN4196810.2941284543953089No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACN3927740.27527100237242824No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCN3741420.26221298601644977No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGAN3689960.25860647291169103No Hit
AAGCTACCATCTGTGGGATTATGACTGAACGAGATN3685660.2583051125084562No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAN3605050.2526556562050244No Hit
CATTTATCAGATCAAAACCAACAGATCGGAAGAGCN3568500.2500940927775286No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCN3552340.2489615383318834No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACN3527110.2471933236868569No Hit
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCN3432940.24059353085601484No Hit
TATCAGATCAAAACCAACCAGATCGGAAGAGCACAN3409800.2389717913837234No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCN3259550.22844169822418195No Hit
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCN3110480.2179943039659933No Hit
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAN3099670.21723669792902398No Hit
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAAN2936580.2058067285886605Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAGAN2857370.20025538962581668No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCCAGN2847460.19956085902208257No Hit
TATCAGATCAAAACCAACAGATCGGAAGAGCACACN2797710.19607418924047063No Hit
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCAN2666090.18684975754889763No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGN2576900.1805989821152903TruSeq Adapter, Index 1 (97% over 35bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGN2551480.1788174515454697No Hit
CGTGCATTTATCAGATAGATCGGAAGAGCACACGTN2455510.17209150784815727No Hit
TTTTGTACGTAGCAGAGCAGCTAGATCGGAAGAGCN2415650.16929796699398542No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGAN2324210.1628895029772901No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGN2323860.16286497364214308No Hit
CGTGCATTTATCAGATCAAAACCAACAGATCGGAAN2319600.1625664165914965No Hit
GCATTTATCAGATCAAAACCAACAGATCGGAAGAGN2283980.1600700311116771No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATN2280600.15983314781797162No Hit
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTN2244770.157322044737064Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCCAGAN2232970.15649505572353595No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGN2217510.15541155994818479No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGN2192960.1536910022971582No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGN2183900.15305604293592398No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGAN2141990.1501188302616053No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAN2134900.1496219360153414No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCN2130370.14930445633472428No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTN2118230.14845363882419627No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGN2025790.1419750909927952No Hit
TTTTGTACGTAGCAGAGCAGCTCAGATCGGAAGAGN2022710.14175923284350142No Hit
ATTAATGATGAGATATAACCTTGACTGAAGAGATCN2016020.14129037212311985No Hit
CATTTATCAGATCAAAACCAACCAGATCGGAAGAGN1981100.13884304531359448No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGN1980430.1387960891577416Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGGATACCGCAGCTAGGAATAATGGAATAGATCGGN1886410.13220680889910544No Hit
TCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGN1847820.12950227448960988No Hit
CTACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCN1804030.12643330424364435No Hit
CGGGGGGGATGCGTGCATTTATCAGATAGATCGGAN1783960.12502672208249963No Hit
TTGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACN1779900.12474218179479421No Hit
TACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACN1756510.12310292136882633No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACN1736490.12169984339841688No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGN1696910.11892592601236263No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAN1684020.11802254564080529No Hit
AAAAGGGACAAGCATCAAGCACGCAGCAGATCGGAN1645490.11532221626019211No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCN1631240.11432352190063494No Hit
TACGCCTGTCTGAGCGTCGCAAGATCGGAAGAGCAN1608880.11275644780381398No Hit
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGN1590900.1114963408154043No Hit
GGGATGCGTGCATTTATCAGATCAGATCGGAAGAGN1567130.10983044853984823No Hit
AGATCAAAACCAACAGATCGGAAGAGCACACGTCTN1543500.10817436799835096Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTGCATTTATCAGATCAGATCGGAAGAGCACACGN1541160.10801037187193946No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGN1511470.10592958341332526No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAN1497870.10497644353332683No Hit
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAAN1466320.10276529917935988Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN1464620.10264615669436009Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACN1452290.10178202325903797Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)