FastQCFastQC Report
Fri 30 Jun 2023
SRR1803156.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803156.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124064324
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAN47096273.7961170851984813No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAN22662591.8266806499505854No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCN21991241.7725675916309347No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACN19153791.5438596191440175No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN18316751.4763913919363314Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATN15522151.2511372729520533No Hit
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACAN14425281.1627258775858884No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAN13680461.1026908912186553No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAN12300090.991428446424292No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATN10411510.8392025736584837No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGN9830550.7923752520507024No Hit
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACN9311130.7505082605374935No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCN9112810.7345230043731186No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCN8814250.710458068509687No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATN8382020.675618882991697No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACN8320020.6706214753566061No Hit
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAN7349400.5923862527957674No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCN6857550.552741495613195No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCN6847600.5519394922911118No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAN6242130.5031365826004903No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGN5998860.4835282059006745No Hit
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACN5548430.44722203943173866No Hit
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCAN5396630.4349864510606611No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGN4958480.39967009371686896No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCAN4909580.39572858995306337No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTN4854430.39128331525830096No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCN4434410.35742829663102826No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCN4272620.34438748080390946No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCN4070060.3280604664399735No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTN3898510.3142329619270726No Hit
CGTGCATTTATCAGATAGATCGGAAGAGCACACGTN3795070.305895351511366No Hit
CATTTATCAGATCAAAACCAACAGATCGGAAGAGCN3697170.29800428364885945No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATN3665370.2954410971521515No Hit
AAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGN3641110.2934856599065498No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCN3609440.29093295184520573No Hit
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCAN3408670.2747502174758958No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACN3257150.2625371980425251No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGAN3193010.25736729924067453No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATN2951390.23789191806663132No Hit
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACAN2943530.2372583757438601No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAN2855120.23013223366291827No Hit
TTTATCAGATCAGATCGGAAGAGCACACGTCTGAAN2757210.2222403597669222Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCN2740200.2208692968012303No Hit
CGTGCATTTATCAGATCAGATCGGAAGAGCACACGN2645360.21322487518652017No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACN2632730.21220685488924276No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGN2624550.21154751949480657No Hit
TATCAGATCAAAACCAACCAGATCGGAAGAGCACAN2596110.20925516025058097No Hit
TATCAGATCAAAACCAACAGATCGGAAGAGCACACN2567550.206953128604481No Hit
ATTTATCAGATCAAAACCAACCAGATCGGAAGAGCN2532750.2041481320609138No Hit
ATTAATGATGAGATATAACCTTGACTGAAGAGATCN2481300.20000108975727787No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTAGATCGGAN2479620.19986567613103667No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGN2447760.1972976534333915No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCN2408440.19412832975255642No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAN2332190.18798232439488405No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGAN2331820.18795250115577142No Hit
GCATTTATCAGATCAAAACCAACAGATCGGAAGAGN2315910.1866701018739279No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGN2306710.18592855106355957No Hit
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAAN2131870.17183586153260305Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAN2131290.17178911159021026No Hit
TACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACN2127740.1715029697014268No Hit
CATTTATCAGATAGATCGGAAGAGCACACGTCTGAN2076050.1673365825940421Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAN2054070.1655649209840534No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAN2022090.1629872258845339No Hit
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACN1984850.15998555716952118Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCN1969760.15876925263381922No Hit
CCAAGTCCTTCTGATCAGATCGGAAGAGCACACGTN1914270.1542965728004128No Hit
CCAAGTCCTTCTGATCGAAGATCGGAAGAGCACACN1906890.15370172008513905No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGN1902660.15336076791906753No Hit
CATTTATCAGATCAAAACCAACCAGATCGGAAGAGN1848670.14900899310909071No Hit
CAAGTCCTTCTGATCAGATCGGAAGAGCACACGTCN1837440.14810381750034765No Hit
CGTGCATTTATCAGAGATCGGAAGAGCACACGTCTN1759910.14185463985601532Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGN1734900.1398387500986988No Hit
AAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTN1723420.13891342365271744Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAGCTACCATCTGTGGGATTATGACTGAACAGATCN1705510.13746981767296776No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGN1672620.13481877352590096No Hit
CGTGCATTTATCAGATCAAAACCAACAGATCGGAAN1666620.1343351534321825No Hit
CATTTATCAGATCAGATCGGAAGAGCACACGTCTGN1645310.13261749606599235Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTAGACGACCTGCTTAGATCGGAAGAGCACACGTN1637220.13196541497296194No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTN1566110.12623371082890839Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATN1557680.12555422459723395No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGAN1543710.1244281958123594No Hit
CAAGTCCTTCTGATCGAAGATCGGAAGAGCACACGN1471670.11862153055377951No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGN1467410.11827816028723939No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN1405640.11329929142240762Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACN1352120.10898540018643876No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAGATCN1341190.1081044055823816No Hit
GTGCATTTATCAGATCAAAACCAACAGATCGGAAGN1324560.10676397188929188No Hit
CTACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCN1291940.1041346906464424No Hit
GGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAGN1282230.10335203212810799No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAN1279620.10314165738734046No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAN1279550.10313601515291373No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAN1250720.10081222060259645No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAN1244400.10030280743721298No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGN1243260.10021091961940647No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)