FastQCFastQC Report
Fri 30 Jun 2023
SRR1803157.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803157.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103804301
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC29765532.867465963669463No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT26551192.5578121276497012No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG19662491.8941883727919904No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT16047121.5459012628002764No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAC14390841.3863433269494296No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG13645501.3145409071248406No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG11155681.0746837936898201No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATC10746081.0352249277224073No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA10084580.971499244525523No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC9821170.9461236100419385No Hit
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACG8771810.8450333864297203No Hit
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACAC8707640.8388515616515735No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATC8654400.8337226797567858No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAT7951220.7659817486753271No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAA7814940.7528531982504271Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA7470710.7196917592075496Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT7332440.706371501889888Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAC6987660.6731570785299157No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG6594780.635308935802188No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT6469100.62320153767039No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA6252700.6023546172715907No Hit
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACA4885570.47065198194437047No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT4746390.45724405966569726TruSeq Adapter, Index 1 (100% over 35bp)
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCA4739110.4565427399776046No Hit
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAAC4624380.44549021143160533Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCA4619490.44501913268507054No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGA4586820.44187186424963254Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGACCCGGG4352390.41928802160134004No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC4040410.3892333902426644No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGAT3824950.36847702485853645No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACA3574750.3443739773364497No Hit
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCT3555890.3425570969357041Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT3458880.33321162675138094No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCA3378210.32544027246038676No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGA3348830.32260994657629843TruSeq Adapter, Index 1 (100% over 36bp)
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCT3127870.3013237380212213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGA3093260.2979895794491213No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCG3066240.29538660445293113No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACA3020470.29097734591941427No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG2913210.2806444407346859No Hit
AAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGT2895900.278976879773026No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCCAGAT2822410.2718972116579254No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATC2808510.2705581534622539No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCG2737600.2637270299618895No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCG2730550.2630478673518547No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCAC2628980.25326310901125376No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACA2508860.24169133415772437No Hit
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAA2424620.2335760634812232No Hit
TGGATACCGCAGCTAGGAATAATGGAATAGATCGGA2288950.22050627748073753No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA2272440.21891578461667016No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC2260230.21773953277716304No Hit
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTG2195430.21149701687216216Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAAC2183940.21039012632048842Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA2065450.19897537771580387No Hit
TACGCCTGTCTGAGCGTCGCAAGATCGGAAGAGCAC2046870.19718547114921567No Hit
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCAC1968010.1895884834290248No Hit
GCAGAGCAGCCAGATCGGAAGAGCACACGTCTGAAC1939750.18686605288156605Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGA1896800.18272845939206314No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGA1868580.18000988224948405No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGA1830090.1763019434040599No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCG1711570.1648843047457157No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA1693440.1631377489840233No Hit
GTTTCGTACGTAGCAGAGCAGATCGGAAGAGCACAC1690280.16283332999853253No Hit
CTACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCA1642350.15821598760151565No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG1625550.156597557552071No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCCAGA1584120.15260639344799404No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGAT1502250.14471943701061096No Hit
CGTGCATTTATCAGATAGATCGGAAGAGCACACGTC1494310.14395453614200437No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCA1482380.14280525813665468No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1474500.14204613737536753No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1464640.1410962730725387Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGC1458270.14048261834545758No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1453640.14003658673064037Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACGCCTGTCTGAGCGTCGCTGCAGAGCAGCTCAGA1442550.13896823022776292No Hit
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1428350.1376002714954942Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAGAT1407140.1355570035580703No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGA1397910.13466783038209562No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGAT1397260.13460521255280164No Hit
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCAC1374490.13241166182507216No Hit
TAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTG1345580.12962661344831944Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATTTATCAGATCAAAACCAACAGATCGGAAGAGCA1331040.12822590077457388No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGAT1327050.12784152363783077No Hit
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTC1324860.12763054972067103No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGG1306600.12587147039311986No Hit
GGGATGCGTGCATTTATCAGATCAAAACCAACAGAT1297640.1250083077000827No Hit
TCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTC1271600.12249974112344343No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGG1267470.12210187706962163No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAC1247680.12019540500542458No Hit
ATTTATCAGATCAAAACCAACCAGATCGGAAGAGCA1227280.11823016851681319No Hit
AAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTG1224690.11798066055085712Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTAGGAATAATGGAATAGATCGGAAGAGCACACGT1201190.1157167851840744No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGG1187150.11436424007132422No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGG1184050.11406560119315287No Hit
CAAGTCCTTCTGATCAGATCGGAAGAGCACACGTCT1180830.11375540210034264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACT1170350.11274581002187953Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1164670.11219862652897204No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGC1151460.11092603956747418No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGA1137630.10959372483034205No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGA1124710.1083490750542215No Hit
TCGTACGTAGCAGAGCAAGATCGGAAGAGCACACGT1095390.10552452927745258No Hit
GGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAGA1092530.10524901082855903No Hit
GAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG1088870.10489642428207287Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACA1058320.1019533863052553No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGG1049220.10107673669513945No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGAT1040880.10027330177773655No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)