FastQCFastQC Report
Fri 30 Jun 2023
SRR1803159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102081758
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT35449873.4726939165761626No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAA24102572.3611045178120857Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT23529722.304987733459684No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG20466952.0049566544494657No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGA18290081.7917089554825258TruSeq Adapter, Index 1 (100% over 36bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG16082961.5754979454801317No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC15986141.5660133909527696No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG15793191.5471118747778618No Hit
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAAC13034961.2769137459407782Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC11894571.1652003485284805No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG11540811.1305457729284012No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT8092700.7927665195577842TruSeq Adapter, Index 1 (100% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATC7822700.766317131803314No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAT6787820.6649395673612909No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGA6099720.5975328128655465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA6031600.590859730295789No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGA5993260.5871039172346543No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT5583840.5469968493293386Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA5407080.5296813168127453Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA5229270.5122629255659958No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACA5097790.49938305333652266No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA5017520.49151974831781403No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG4877400.4777934956801978No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC4752480.4655562456124629No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGA4446630.4355949669283713No Hit
GCCCGGATAGCTCAGATCGGAAGAGCACACGTCTGA4359590.42706846800189313Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC4229400.41431496506946913No Hit
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAAC3813460.3735691934302307Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATC3723890.3647948539444236No Hit
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAC3683000.3607892411100522No Hit
GCCCGGCTAGCTCAGATCGGAAGAGCACACGTCTGA3615510.3541778737783885Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGG3438620.336849606371395No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCA3435210.33651556040012554No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG3425550.33556926008268784No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGAT3096120.3032980682013725No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGATCGGAA3058500.29961278684091625No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCAC2992800.29317676915399515No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA2961180.29007925196586054No Hit
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACG2931050.2871276962138524No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCG2914530.2855093855260604No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT2415530.2366269985279838Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG2344630.2296815852250507Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATC2339960.22922410877759375No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2330030.22825135907240157No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGA2298020.22511563721306604No Hit
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACAC2207670.21626488838485716No Hit
TAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTG2173550.2129224694582552Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGC2161260.2117185325119499No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGAT2124750.20814198752337315No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGA2078160.20357799872529628No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT2052640.2010780417790219No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAC2051540.20097028501409622No Hit
CCCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2007920.19669723948131848Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAA1990680.19500839709284787No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGAGATCGGAAG1985150.19446667444735816No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGA1925150.18858903272414254No Hit
GAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG1924270.18850282731220205Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGAT1879730.1841396579396683No Hit
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC1835380.17979510109925811Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGAT1724370.16892048430435533No Hit
TTTATCAGATCAGATCGGAAGAGCACACGTCTGAAC1692810.1658288447579439Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT1666900.16329068314046863No Hit
GCAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA1658840.16250111993565Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGC1649810.16161653485630606No Hit
GTTCAGAGATCGGAAGAGCACACGTCTGAACTCCAG1631600.15983267059331013Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC1629930.15966907623201396Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCG1621870.15887951302719533No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACA1610590.15777451638323078No Hit
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTC1531880.15006403004932575No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCA1515310.14844082132676437No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCG1504790.1474102748112939No Hit
CGTGCATTTATCAGATAGATCGGAAGAGCACACGTC1461110.14313135163679294No Hit
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACT1450910.14213215254384629Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTC1444910.14154438837152472No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCA1444640.14151793898377024No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCAC1432600.14033849221131164No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCA1424270.13952247961873854No Hit
GGGATGCGTGCATTTATCAGATCAAAACCAACAGAT1409650.13809029425218167No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGG1380450.1352298419468834No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACA1349770.1322244078124125No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCG1349210.1321695498229958No Hit
GATGGAATAGATCGGAAGAGCACACGTCTGAACTCC1333850.1306648735418526Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCAC1308250.12815707973994728No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1305160.12785438119120165No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATC1275200.12491947875740933No Hit
TGGGAAACTCGACTGCATAATTTGTGGTAGTGGAGA1271010.12450902344373811No Hit
AGACTCTGGCATGCTAACTAGTTACGCGACCCCAGA1245000.12196106575672414No Hit
CGTGCATTTATCAGATCAGATCGGAAGAGCACACGT1229470.12043973615736515No Hit
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAAC1210610.11859219744236771Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGA1194520.11701600985359206No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1185290.11611183263517072No Hit
AAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGT1172670.11487556865938771No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA1164530.11407816859893812Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTAGAT1148750.1125323488257324No Hit
GCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT1143990.11206605591569065Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAC1107990.10853947088176126No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1106520.10839546865954248Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAGAGCAGCCAGATCGGAAGAGCACACGTCTGAAC1100970.10785178680014504Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGG1082140.1060071869060092No Hit
CGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACA1077930.10559477237843025No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1074120.10522154212900606Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGA1073570.10516766374654322No Hit
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCAC1072660.10507851951374113No Hit
GCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1063630.10419393443439717Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA1058860.10372666191740154Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)