FastQCFastQC Report
Fri 30 Jun 2023
SRR1803161.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803161.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140170765
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCN37743252.6926620540310244Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN30770942.195246633632912Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAN14548721.0379282727036554Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGN14009630.9994687551287887No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGN13875540.989902566344701No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACN13252980.9454881693768312No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN12386580.8836778482303353Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATN11273950.8043010965945716TruSeq Adapter, Index 1 (97% over 48bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACN9361030.6678304138527031No Hit
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN8639530.6163574836735749Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN8570180.6114099470028576TruSeq Adapter, Index 1 (97% over 35bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTN8459730.6035302725215204No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCN8351020.5957747323416548Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN8288680.591327299954452Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN7906700.5640762537038305TruSeq Adapter, Index 1 (97% over 37bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACN7394480.527533683646515No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTN7007370.4999166552312103No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTN6455680.4605582340939639Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN6446410.45989689790164157Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTN6238050.445032172008193Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACN6094630.4348003665386288No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCN5948990.4244101828223596No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTN5466070.38995792025534No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCN5170440.3688672170691228No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCN4372230.3119216763923633Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN4362030.31119399255615104Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN4123700.29419116033218484Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACN3941900.28122126607499076No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAN3754400.2678447249681487Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGN3730950.2661717655603863No Hit
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN3710460.2647099771482306TruSeq Adapter, Index 1 (97% over 35bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTN3587210.2559171307939997No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGN3520590.25116435656179803Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCN3516110.25084474640628524TruSeq Adapter, Index 1 (97% over 36bp)
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN3508680.25031467867069146TruSeq Adapter, Index 1 (97% over 37bp)
TGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTN3468070.24741749822083087Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAN3448390.2460134964662567Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGN3378870.24105383173160252Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN3352780.2391925306250558Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCN3235870.23085198971411763Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATTAATGATGAGATATAACCTTGACTGAAGAGATCGGAAGAGCACACGTN3228220.23030622683695845No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAN3199820.22828012674397544Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCN3199670.22826942551109No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAN3193400.22782211397647717TruSeq Adapter, Index 1 (97% over 47bp)
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAN3133500.2235487549775447TruSeq Adapter, Index 1 (97% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCN3098000.22101612986131594No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTN3069670.21899502367701282Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCN2957200.21097123925948466Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAN2569620.18332068031447213No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCN2486830.17741431317721637Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGN2442710.1742667238778357TruSeq Adapter, Index 1 (97% over 39bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACN2426250.1730924419225364No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN2411460.1720373003600287TruSeq Adapter, Index 1 (97% over 35bp)
ATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATN2389140.17044495690667025TruSeq Adapter, Index 1 (97% over 41bp)
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCN2379970.16979075486960493Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN2285210.1630304293480884TruSeq Adapter, Index 1 (97% over 37bp)
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCN2267840.16179122657995051Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCN2222820.15857942988325704Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGN2210180.15767767265877447No Hit
GAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCN2186890.15601612789942323TruSeq Adapter, Index 1 (97% over 42bp)
GGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAN2124170.15154158572224385Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN2117810.15108785344789977TruSeq Adapter, Index 1 (97% over 35bp)
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCCN2099140.14975590666142116Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN2088450.14899326546444971Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN2083170.14861658206688108TruSeq Adapter, Index 1 (97% over 37bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACN2054500.1465712197547042No Hit
TTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCN2042890.14574294432936852Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTN1989240.14191547003399746No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACN1981140.1413376034581819No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTN1935260.13806445302627834No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAN1910130.13627163981019866No Hit
CTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCN1903260.13578152334404395Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAAN1884830.13446669853018212Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGN1868770.1333209531959107No Hit
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN1863320.13293214173440518Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATN1821430.12994364409725523TruSeq Adapter, Index 1 (97% over 41bp)
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCN1797020.1282021967990258Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAN1731350.12351719704176545Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCN1710690.12204328056567289TruSeq Adapter, Index 1 (97% over 36bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAN1708060.12185565228241424No Hit
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN1707290.12180071928693548TruSeq Adapter, Index 1 (97% over 37bp)
CCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCN1696990.12106590129546627Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGN1645090.1173632747170924Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGN1642890.11720632330143878No Hit
GATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACN1632830.11648862728258634TruSeq Adapter, Index 1 (97% over 38bp)
TTCTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAN1582580.11290371426595268TruSeq Adapter, Index 1 (97% over 40bp)
TATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTN1566870.11178293847508072Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTN1562920.11150113934242994No Hit
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCN1514680.1080596228464616Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTN1491240.1063873768542249No Hit
GTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN1488560.10619618149333779Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTAGATCGGAAGAGCACACN1485380.10596931535616576No Hit
AAATACCACTACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN1436250.10246430487841028Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTN1434540.10234231082351587Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACN1421660.10142343162641654Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TTTATCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN1406230.10032263147026414TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)