FastQCFastQC Report
Thu 6 Jul 2023
SRR1803162.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803162.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120339756
Total Bases12.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA58848084.8901611533930645Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA32887892.7329197842149524Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG20163731.6755668010495217Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA15368591.2770999801595078Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT14466961.2021762783032401No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT13105521.089043258488907No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC12406921.030990955308236Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG9599110.7976673976304224No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG9318550.7743534065334153TruSeq Adapter, Index 1 (100% over 49bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC9149350.7602932151532699Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC8897740.7393849128296387No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG8686410.7218238002742834Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC8683360.7215703511979865No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT7995920.6644454223423887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA7798240.6480185982760344Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT6985280.5804632012050946TruSeq Adapter, Index 1 (100% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC6893680.572851419110406No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT6650670.5526577600838746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT6203430.5154929847123838Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5619100.46693629659677893Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT5591450.46463863529854593Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC5539450.46031753629282746Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC5217670.43357824325321054TruSeq Adapter, Index 1 (100% over 38bp)
ATTAATGATGAGATATAACCTTGACTGAAGAGATCGGAAGAGCACACGTC4817870.40035563974385985No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT4609670.38305462410942565TruSeq Adapter, Index 1 (100% over 35bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG3881390.32253597057318284No Hit
CGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3778410.31397853258070424Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA3748710.31151052026397663Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCA3276150.2722417020689322Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT3269840.27171735332419983TruSeq Adapter, Index 1 (100% over 48bp)
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT3212300.2669358910782568Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT3197490.26570520884220505Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC3180240.26427176734511576No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG3133240.2603661586284087No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG3121610.25939972821616825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3103300.25787820277780854Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3020290.25098023299964145Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2954980.24555309884457469Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC2948750.2450353979444665TruSeq Adapter, Index 1 (100% over 36bp)
AAATACCACTACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2865270.23809837207913234Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2829010.23508523650322177No Hit
TTCTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT2816760.23406728529514384TruSeq Adapter, Index 1 (100% over 41bp)
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCA2812070.2336775554040512Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA2745370.22813491494863927TruSeq Adapter, Index 1 (100% over 37bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2621370.21783075578115682No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCC2603220.2163225260320455Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAG2593700.21553143252176782Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC2537980.21090120874102486TruSeq Adapter, Index 1 (100% over 36bp)
ATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA2512320.2087689125778184TruSeq Adapter, Index 1 (100% over 40bp)
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2508760.2084730834920423Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC2441250.20286313360981054No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT2378850.19767781480294844Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAA2370830.19701136837937416Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2330230.19363758723260166TruSeq Adapter, Index 1 (100% over 35bp)
TTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTC2306230.19164323384534698Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT2302590.1913407569149467No Hit
ATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACT2278040.1893006995959008Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG2225860.18496464293977793Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA2039610.1694876296741037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA2034120.1690314213367692TruSeq Adapter, Index 1 (100% over 37bp)
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT1986730.16509340437751927TruSeq Adapter, Index 1 (100% over 41bp)
ACTACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA1985800.16501612318376313TruSeq Adapter, Index 1 (100% over 40bp)
GAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG1968570.1635843436478299TruSeq Adapter, Index 1 (100% over 39bp)
CTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1964240.16322452905754603Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC1935870.1608670371576954TruSeq Adapter, Index 1 (100% over 38bp)
TGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGA1935560.16084127675977672Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT1924130.15989146595909667TruSeq Adapter, Index 1 (100% over 41bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1917300.15932390622430712No Hit
GGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1899750.15786553530987713Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA1836510.1526104141344611Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG1811200.15050720229148545TruSeq Adapter, Index 1 (100% over 39bp)
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGT1797410.14936128007439203Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1774290.14744005297800336No Hit
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1754310.14577975378311386Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT1747570.14521967287352652TruSeq Adapter, Index 1 (100% over 43bp)
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1712310.14228963535541822Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAAC1691040.14052213966596375Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1680980.13968617320447282Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT1652370.13730873777074967No Hit
TGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1557120.12939364776508272TruSeq Adapter, Index 1 (100% over 36bp)
TCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1495660.12428644113255474Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1490660.12387095084354334Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC1451110.12058442265746325TruSeq Adapter, Index 1 (100% over 42bp)
CTTCTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA1445380.1201082707862562TruSeq Adapter, Index 1 (100% over 40bp)
TTTCGTACGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC1387880.11533013246262523TruSeq Adapter, Index 1 (100% over 38bp)
CTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1350230.11220149058636948TruSeq Adapter, Index 1 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA1340350.11138048177528298Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1335510.11097828717551994Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCA1316400.10939028329091842Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1313340.10913600323404346No Hit
TTTATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG1309610.10882604747844096TruSeq Adapter, Index 1 (100% over 39bp)
AAATACCACTACTCTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1270820.10560267381629061Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1263610.10500353681953617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1257580.10450245553098846Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC1233580.10250810214373378Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGGAAGAGCACACGTCT1231410.10232777935830284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCC1228420.10207931616547403Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT1224150.10172448745865831TruSeq Adapter, Index 1 (100% over 43bp)
TTTATCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC1223090.10163640351738788TruSeq Adapter, Index 1 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1218970.1012940395192425No Hit
GCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAG1216050.10105139319045985Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCGGTGGATCACTCGGCTAGATCGGAAGAGCACACGTCTGA1216000.10104723828756974Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)