FastQCFastQC Report
Fri 30 Jun 2023
SRR1803163.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803163.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123597623
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCN51266204.147830577615559No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATN30187922.442435320944643No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCN26537232.147066371980309No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCN25479072.0614530750320337No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN24029171.9441449937916684Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAN23771491.9232966964097684No Hit
ATTGATCATCGACACTTCGAACGCACTAGATCGGAN21868451.7693260978004406No Hit
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCN10731590.8682683161309663No Hit
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGN10241140.8285871322946072No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAN6953650.5626038617263699No Hit
AGAGGTGTAGAATAAGTGGGAGGCCCCAGATCGGAN6836080.553091542868911No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTN6055480.489934988474657No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGAGATCGN6029760.4878540423062991No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATN5836500.47221781926987383No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGN5829920.4716854465720591No Hit
AGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAGATN5795460.46889736706344265No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGN5607730.4537085636347553No Hit
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGN5596940.4528355694995849No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCN5444610.44051089882205907No Hit
ATACCACCCTGAACGCGCCCGATCTCGTCAGATCGN5338060.43189018287188263No Hit
ATTGATCATCGACACTTCGAACGCACTTAGATCGGN5016250.4058532743789094No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGN4980750.4029810508572645No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGN4969230.4020489940975645No Hit
CGACTCTTAGCGGTGGATCACTCGGCTAGATCGGAN4966090.40179494390438236No Hit
ATTGATCATCGACACTTCGAACGCACTTGAGATCGN4796070.3880390159283241No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAN4778040.38658025001014784No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGN4517800.3655248289038698No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGN4392230.3553652484077303No Hit
AAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAN4318950.3494363317974165No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGAGATCGN4232020.34240302501610403No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAN4204250.3401562180528342No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAN4010440.32447549577874973No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAGATN4000270.32365266442057705No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCN3981130.3221040909500339No Hit
AGTCGGCATTGGCAATTTTTGACAGTCTCTAGATCN3961820.3205417631696687No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCAN3911890.3165020414672538No Hit
AAATGGATTTTTGGAAATAGGAGATGAGATCGGAAN3860960.31238141206000375No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCN3859890.31229484081583025No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAGAN3456420.2796510091460254No Hit
AAATGGATTTTTGGAAATAGGAGATGGAGATCGGAN3353200.27129971585294965No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAN3343030.270476884494777No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTN3284150.26571303883408826Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAATCGGTCGTCCGACCTGGGTATAGGGGCAGATCN2969940.24029102889786155No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTAGATCN2829650.22894048698654987No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGAGATCGGN2815400.2277875521926502No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAN2812550.22755696523387026No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGATCGGAN2778460.22479882157604275No Hit
AGCTGCGAGAATTAATGTGAATTGCAGATCGGAAGN2730070.22088369773907382No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAAAGATCGN2683480.217114207770808No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATN2626200.21247981443785532No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAGATN2624730.21236088011174778No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCN2584350.20909382699050774No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGN2568260.2077920220197115Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGN2477510.20044964780592905No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAN2445040.19782257462993444No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGAGATCGN2347910.1899640092593043No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAN2321470.1878248095434651No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACN2316780.18744535240778862No Hit
AGAGGTGTAGAATAAGTGGGAGGCCCCCAGATCGGN2290350.18530696176899777No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTN2152580.17416030727387047No Hit
ATTAATGATGAGATATAACCTTGACTGAAGAGATCN2134980.17273633166877328No Hit
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGN2132320.172521117173912No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGAN2089530.16905907648401944No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGN2059310.1666140456439037No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGN2055840.16633329590812598No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAN2046520.16557923609906317No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGN2026960.16399668139248924No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAN1988590.16089225275796767No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGN1975610.1598420707492085TruSeq Adapter, Index 1 (97% over 35bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGAN1937950.15679508658511984No Hit
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGN1915490.1549778995345242No Hit
GGGACATGTGGCGTACGGAAGACCCGCAGATCGGAN1904620.15409843278296703No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGATCGGN1886600.1526404759418391No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGAGATCN1870520.15133948004809122No Hit
ACGAATTTTTGAGCGGGTAAAGGTCGCCCTCAGATN1848640.14956921946629995No Hit
ATATTAGTCAGCGGAGGAAAAGAAACTAGATCGGAN1759370.1423465886556734No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGN1732110.14014104462186946No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATN1731540.1400949272301135No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGGTAGATCGGN1674590.13548723343975638No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATN1667690.1349289702763944No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGN1667370.13490307981084718No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCN1663080.13455598575710473No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATN1652820.13372587270549693No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGATCGGAN1651640.13363040161379156No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTTAGAN1648430.133370687881271No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATN1637670.13250012097724564No Hit
ATTGATCATCGACACTTCGAACGCACTTGCAGATCN1625730.13153408298151495No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGAN1607750.13007936244858043No Hit
AAATGGATTTTTGGAAATAGGAGATGGAAGATCGGN1550250.125427169420564No Hit
ATATTAGTCAGCGGAGGAGAAGAAACTAGATCGGAN1444920.11690516087028632No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGAGATCN1407480.11387597640126136No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCN1370440.11087915501417046No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGN1365270.11046086218017316No Hit
CGCTCCCCGCCCCCGGAGAGATCGGAAGAGCACACN1351020.10930792738627344No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGAGAN1350170.10923915583716363No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAN1325150.10721484506219024No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACN1293530.1046565434353054No Hit
TGATCCTTCGATGTCGGCTCTTCCTATCAGATCGGN1288510.10425038675703335No Hit
TAATGATGAATGCCAACCGCTCTGATGAGATCGGAN1283250.1038248122296009No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAN1272890.10298660840750958No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGN1270620.10280294791753397No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGN1257790.10176490206450005No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCN1250030.10113705827497993No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)