FastQCFastQC Report
Fri 30 Jun 2023
SRR1803165.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803165.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142945077
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCN70746304.949194577718825Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN37747242.640681357637801Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGN32154072.2494003063848083No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAN29940642.09455551939015Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTN23933331.6743025015125215Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACN22388011.5661966448834053No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTN21792121.5245100046362563No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGN21152821.4797865336768472No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTN20861241.459388489468581No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCN19817161.3863478488314782No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCN18471701.2922235859861055No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTN18297521.280038486390126No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN18167121.2709161015737536Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCN16080981.124976133315875Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATN12680810.8871106487983492TruSeq Adapter, Index 1 (97% over 48bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTN11827010.8273814144715177Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGN10011250.7003564033198569Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGN8922650.6242012797684526Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATTAATGATGAGATATAACCTTGACTGAAGAGATCGGAAGAGCACACGTN8030080.5617598149252807No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGN7763290.5430960032292683No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACN6909380.4833590736391712No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN6823270.47733508164118166Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACN6769270.47355740694728504No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN6271280.4387195510062931Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTN6008240.42031807783069014No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCN5684130.3976443344040453Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN5411330.3785600815059899Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGN5029630.351857517975243Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCN4828390.3377793836159884Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACN4817150.3369930676241477No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTN4749590.3322667768404504No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGN4634050.3241839521342872No Hit
CGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAN4463130.31222691215871673Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTN4362360.305177351438273Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN4211140.29459846315658705Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCN4106240.287259980278999No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGN4070260.2847429296218435No Hit
CTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCN4018550.2811254563177436Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCN4000950.2798942141952884No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGN3977070.27822364249732084No Hit
TAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGN3962170.2771812841095605Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN3858190.26990716161564626Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGACTCTTAGCGGTGGATCACTCGGCTAGATCGGAAGAGCACACGTCTGN3790400.26516478073603056Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCN3720570.26027968770131205Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCN3697950.258697261746202No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACN3681900.2575744528788494No Hit
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTN3626100.25367085569515624Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTN3510720.2455992240991972Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN3454480.24166484586244266TruSeq Adapter, Index 1 (97% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCN3406140.23828312744201746No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN3336240.23339313742158468Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN3262610.22824220801951786TruSeq Adapter, Index 1 (97% over 37bp)
TGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCN3193360.22339769000928938TruSeq Adapter, Index 1 (97% over 36bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTN3192950.22336900766439124Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTN3140530.2197018649337605No Hit
GGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAN3048250.2132462386235239Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCN2800010.19588012814180372Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTAGATCGGAAGAGCACACGTN2773350.19401507615403923No Hit
CCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTN2725480.1906662374948387Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN2717800.19012896820504002TruSeq Adapter, Index 1 (97% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTN2683710.1877441361621709No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTN2660080.18609105369889722Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN2566920.17957386528253785Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTN2429820.16998276897636705Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACN2422680.1694832764335074Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGN2310700.16164949842938628Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAAN2260830.15816074589263399Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGGAAGAGCACACGTCN2176900.15228926002117582No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGN2175390.15218362504362426No Hit
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN2144510.15002335477422563Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTN2106200.14734330444972232No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACN2093730.1464709414231873No Hit
GTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN2078500.14540549724563093Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTN2060060.14411549129460402Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTN2057900.14396438430684816Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN2000140.13992367152315432Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACTACTCTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN1988230.1390904843823338TruSeq Adapter, Index 1 (97% over 37bp)
GTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTGAACTCN1816480.12707538014757935Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTN1644380.11503579098425334Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAN1629150.11397034680669695TruSeq Adapter, Index 1 (97% over 40bp)
TGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN1606370.1123767277413828TruSeq Adapter, Index 1 (97% over 35bp)
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCN1582260.11069006594749675Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACN1535360.10740908551890876No Hit
AAATACCACTACTCTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTN1526340.10677807393115049Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCN1506670.10540202094542928Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCAGATCGGAAGAGCACACGTCTGAN1482420.10370556518011459Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GCCGCCGGTGAAATACCACTACTCTGATAGATCGGAAGAGCACACGTCTN1481760.10365939360052254Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAN1464960.10248411702908802Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
ATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGN1462840.1023358083188832TruSeq Adapter, Index 1 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)