FastQCFastQC Report
Fri 30 Jun 2023
SRR1803166.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803166.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191637797
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAN23653091.234260170502795No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAN15125820.7892921039997136No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCN13506200.7047774609932507No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATN13271540.6925324861671207No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN12508090.6526943116550228Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACN10591650.5526910748196505No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGN9133680.47661161540069263No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCN8313910.43383456343948684No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTN8144620.42500071110711No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTN7773040.4056110079370199Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAN7417690.3870682149409179No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAN7317560.38184325402154357No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACN7268750.37929626168683206No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATN6370950.33244746598709857No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCN6217910.3244615674641678No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCN5897370.3077352219823316No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAN5699730.297422016388552No Hit
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACAN5660950.29539840723591704No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGN5156090.2690539173751825No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAN5077850.2649712154643481No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTN5070080.26456576308900065No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGN4663340.24334134878413366Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGN4485430.23405768956945378No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAN4329470.22591942026968717No Hit
ATTAATGATGAGATATAACCTTGACTGAAGAGATCN4299700.22436596889078203No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAN3602490.18798431501485063No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCN3590960.1873826591734406No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAN3469190.18102848468874855No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCN3277840.17104350244644065No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATN3268790.17057125740179532No Hit
TACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCAN3218070.16792459788086583No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATN3076750.16055026973619405No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAN2841370.14826772403358404No Hit
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGN2531510.1320986798862022Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACN2504160.13067150839768837No Hit
CGTAGACGACCTGCTTAGATCGGAAGAGCACACGTN2431030.12685545534631668No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCN2398350.12515015500830454No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTN2265230.11820371740132246Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCN2243600.11707502565373365No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGN2206470.11513751642636552No Hit
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCAN2136050.11146287597952297No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATN2122860.11077459839511723No Hit
CGACTCTTAGCGGTGGATCACTCGGCTAGATCGGAN2068120.10791816814717402No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)