FastQCFastQC Report
Fri 30 Jun 2023
SRR1803171.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803171.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140156299
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA78660245.612322853930382No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC46873453.344369845268246No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA33172892.366849741087983Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG32191682.2968414712491803No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT29772092.1242063476576245No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA20954231.4950615954834823Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAG20135741.4366632212512975Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG19678181.4040168112601203Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTC19168051.3676195887564069Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC18510401.320696974168817Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC15277581.0900387716430782No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACAC14794571.055576531740468No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCA12345670.8808501714218353Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGA11887420.848154530678639Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG11457470.8174780642573901Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT11129690.7940913165807839Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGAGC10730660.7656209586413237No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACG10449390.7455526490464763No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG10132740.7229600148046147TruSeq Adapter, Index 1 (100% over 49bp)
TCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCT9919970.7077791059537039Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCA9883570.7051820054124003Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG8868900.632786400845245No Hit
TGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTG7703940.5496677676969767Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC7280300.5194415129354978TruSeq Adapter, Index 1 (100% over 36bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC6879760.4908634181329231No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG6491870.46318788711736747Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGT6450760.46025473318184573No Hit
TCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAG6375160.45486075513452306Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA6252450.44610552965585937TruSeq Adapter, Index 1 (100% over 37bp)
TTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC6154970.4391504373271158Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC6030100.43024109818995715No Hit
TATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC5804950.41417688975933936Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG5386800.38434234054653515No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT5317640.37940784951805845No Hit
CGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTG5255320.37496138507481563Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAA5154110.3677401612895044Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG5068310.3616184242992889No Hit
TTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCC5033770.35915403274168933Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4712420.33622605859476923No Hit
GTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACT4433470.316323278484972Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC4386680.3129848627067414No Hit
ATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAAC4182190.2983947228800612Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA4106570.2929993178544191Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCAC4008530.28600427013273233No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3973750.2835227548353Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCA3961120.2826216180266004Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC3937400.28092922173979495No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC3915500.27936668048005464Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAA3741860.2669776547110451Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA3632720.2591906340220927Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC3617340.258093287694476No Hit
TCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTG3595600.2565421622612909Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCC3444170.24573779591597236Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACG3212260.2291912688133981No Hit
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCCA3197450.22813459136788425Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTC3165410.22584857210020937No Hit
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3136550.22378944238531867Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAG3062350.21849535282035382Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTC3042520.21708050381667113Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACGCCTGTCTGAGCGTCGCTGCAGAGCAGCTCAGATCGGAAGAGCACAC2994960.21368714937314376No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACAC2981040.21269397246284305No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2978430.2125077517921617No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC2969790.21189129715818195No Hit
GCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAG2850700.20339435475532924Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT2769950.19763292979076166Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT2747340.19601973080068275Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCC2740760.19555025493360095Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG2624530.1872573704304221Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC2614390.18653389242248755TruSeq Adapter, Index 1 (100% over 38bp)
GGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGA2571360.18346374856830372Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT2566040.183084172335344Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCGACCGCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC2553600.1821965918206787TruSeq Adapter, Index 1 (100% over 36bp)
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCC2535330.18089304712590906Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGT2504040.17866053954521158No Hit
TACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACT2503770.17864127533789972Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC2491810.1777879423029Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGAGATCGGAAG2416060.1723832619181818No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAGAG2224070.15868498354112504No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACGTC2215360.1580635344830274No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC2205920.15739000071627177Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGA2179160.15548070372491785Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACGCCTGTCTGAGCGTCGCTCAGATCGGAAGAGCACACGTCTGAACTCC2147990.15325675801413677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGAGCAC2136830.15246050411191295No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGAGCA2101730.14995615716137026No Hit
TATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCCAGT2074870.14803972527841933Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC2056460.1467261917354139No Hit
GATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAA2045580.14594991552966163Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2041760.1456773626706567No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC2018640.14402777573343314No Hit
GGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTC2004500.14301890206161907No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT1989060.14191727479904417TruSeq Adapter, Index 1 (100% over 48bp)
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1980740.1413236518181748Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1933750.13797096625675026Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAAAGGGACAAGCATCAAGCACGCAACAGATCGGAAGAGCACACGTCTGA1929230.13764846915656642Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC1903620.13582122341857786TruSeq Adapter, Index 1 (100% over 38bp)
AGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGAACTC1882280.13429863755178068Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1839720.1312620276881027TruSeq Adapter, Index 1 (100% over 35bp)
ACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTC1811810.12927067944338344Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT1807740.128980289355386No Hit
TTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA1803850.1287027420722632Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1797060.12821828293282772Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCA1735030.12379250967521624Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCC1711790.12213436086807629Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAGATCGGAAGAGC1705040.12165275568527961No Hit
TGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1692490.12075732678985765Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAAC1659410.1183971046495741Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTCGGGCCGATCGCACGCCCAGATCGGAAGAGCACACGTCTGAACTCC1594230.11374658230665752Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1550030.11059296022078893Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA1540910.10994225810714364Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACG1501440.10712611639381259No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)