FastQCFastQC Report
Sun 2 Jul 2023
SRR1803173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118605985
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2110999817.798425602215605TruSeq Adapter, Index 1 (100% over 35bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT92583317.805955997920341No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC66301375.590052643633456No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG29851522.5168645578888786No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG24870932.0969371823858634No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG15317281.2914424175137535No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGA11939511.006653247726074TruSeq Adapter, Index 1 (100% over 36bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG9376220.7905351487954002No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC8624890.7271884298250211No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA8613750.7262491854858758No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT7221180.6088377411983047Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA6449590.5437828453597852Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG6280520.5295280841013208Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCG5897730.4972539960778539No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGAT5706870.4811620594019771No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC5110260.4308602133357773No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC5046690.42550045008268345No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATC4877200.41121027745775224No Hit
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACAT4376500.3689948698625959TruSeq Adapter, Index 1 (97% over 35bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAA4316050.3638981624746846No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATC4091630.3449766889925496No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3905320.32926837545339727Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTCGGCATTGGCAATTTTTGACAGTCTCTAGATCG3843200.3240308657273914No Hit
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAA3749110.31609787651103777Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA3684410.3106428398195926No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT3422060.2885233826943893Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAT3055390.25760841664103207No Hit
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGG2856300.240822585807959No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT2848110.2401320641618549No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG2790170.23524698184497184No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATC2513590.2119277538987598No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAC2402760.20258336879036923No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATC2282570.19244981608643105No Hit
GGGACATGTGGCGTACGGAAGACCCGCAGATCGGAA2273250.19166402100197558No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTAGATCG2249260.18964135747449845No Hit
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGA2248170.18954945654723918No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC2061180.1737838103195214No Hit
CGACTCTTAGCGGTGGATCACTCGGCTAGATCGGAA2051170.17293983941872748No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG1977270.16670912517610306No Hit
TCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1976240.1666222830154819No Hit
GCGCTAAACCATTCGTAGACGACCTGCTTAGATCGG1956120.16492590993616385No Hit
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAC1940930.1636451988489451No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCG1928600.16260562230481032No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGA1925280.1623257038841674No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGG1816080.1531187486027792No Hit
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGA1799480.15171915649956452No Hit
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAC1773800.14955400437844682No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1759910.14838289990172082TruSeq Adapter, Index 1 (100% over 36bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCG1744780.1471072475811402No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCG1723080.14527766031368483No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCG1714750.14457533487875845No Hit
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1533340.12928015394838632Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCG1530860.12907105826067714No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA1496380.1261639536993011No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGAGATCGG1450710.12231338915991465No Hit
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACAC1442920.12165659262473139No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATC1377970.11618047773896065No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCA1373170.11577577640791062No Hit
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCAC1344840.11338719542694242No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGA1301620.1097431971919461No Hit
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC1297050.10935788779967553Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAG1259060.10615484538996915No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATC1232930.10395175251906555No Hit
GATCGAGGCCAGATCGGAAGAGCACACGTCTGAACT1220350.10289109778060526Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCTAAACCATTCGTAGACGACCTGCTTAGATCGGA1196430.10087433614753927No Hit
TTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACA1186420.10003036524674534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)