FastQCFastQC Report
Sun 2 Jul 2023
SRR1803174.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803174.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159128523
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAN35234292.214203295282267No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCN31873622.003011113224497No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAN22287621.4006049688527555No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATN17912511.1256630591613046No Hit
CCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACAN17161701.0784804431321215No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAN15880260.9979518253933647No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCN15334970.9636845557851372No Hit
TTTATCAGATCAAAACCAACAGATCGGAAGAGCACN14903040.9365410876087877No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACN12032160.756128428339651No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGN11478340.7213251140400518No Hit
CAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACN10402860.653739493327667No Hit
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCAN10354790.650718664685903No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCN10294520.6469311601666786No Hit
TTTATCAGATCAAAACCAACCAGATCGGAAGAGCAN9838750.6182895319150294No Hit
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAN9622240.6046835487815092No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATN8428020.5296360351437436No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAN7954590.49988461213832797No Hit
CATTTATCAGATCAAAACCAACAGATCGGAAGAGCN7692490.48341364922993724No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCN7358280.46241112914747534No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN6963790.43762047612293864Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAN6365970.4000521012816791No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGN6223960.39112786838347013No Hit
ATTTATCAGATCAAAACCAACCAGATCGGAAGAGCN5744440.36099373586217476No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGN5743060.3609070135088227No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGAN5616610.3529606065658009No Hit
ACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACN5409310.33993340087747814No Hit
TATCAGATCAAAACCAACCAGATCGGAAGAGCACAN4855980.3051608792975474No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN4734780.2975443943509738Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATN4592940.28863084464122124No Hit
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCN4530440.2847032018263627No Hit
TATCAGATCAAAACCAACAGATCGGAAGAGCACACN4259900.2677018500322535No Hit
ATTAATGATGAGATATAACCTTGACTGAAGAGATCN4086110.25678048931554526No Hit
CATTTATCAGATCAAAACCAACCAGATCGGAAGAGN3997020.2511818701415333No Hit
GCATTTATCAGATCAAAACCAACAGATCGGAAGAGN3974200.24974780919697218No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAN3915020.24602880276843891No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCN3724330.23404540743459296No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCN3704450.23279610280804278No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATN3693210.23208975552421862No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGN3572840.2245254296742263No Hit
AAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGN3528330.22172831956719666No Hit
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCN3451360.21689134888784206No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACN3440170.2161881437182698No Hit
AAGCTACCATCTGTGGGATTATGACTGAACAGATCN3430490.2155798303991045No Hit
CGTGCATTTATCAGATAGATCGGAAGAGCACACGTN3323850.20887832912268028No Hit
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCN3283310.20633070288725044No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGN3234200.20324451826904721No Hit
CGTGCATTTATCAGATCAAAACCAACAGATCGGAAN3159910.19857596491359378No Hit
CGTGCATTTATCAGATCAGATCGGAAGAGCACACGN2995340.18823401006493348No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTN2928540.18403614542441268No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCAGATCGN2871050.18042334245759323No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGAN2789970.17532808998673355No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCN2759160.17339191918472088No Hit
GTGCATTTATCAGATCAAAACCAACAGATCGGAAGN2741750.1722978350022139No Hit
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACAN2730580.17159588667834239No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGN2663120.1673565461296967No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTAGAN2433630.15293487013638654No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGN2378730.14948482868781482No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAN2314870.14547172036530495No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGCTN2291030.14397356029000533No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCN2234630.140429255413877No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTN2064610.12974481011176103No Hit
TTTATCAGATCAGATCGGAAGAGCACACGTCTGAAN2043090.12839244413774895Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCAN2027590.12741838871966404No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCN1968570.12370943705673684No Hit
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGN1959000.12310803638892569No Hit
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGN1930830.12133776921941265No Hit
CGGGGGGGATGCGTGCATTTATCAGATAGATCGGAN1924270.1209255238295651No Hit
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCAN1863880.1171304782361362No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGN1817680.11422716466739277No Hit
CCAAGTCCTTCTGATCGAAGATCGGAAGAGCACACN1813040.1139355764648177No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGN1794340.11276042573461202No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATN1699370.10679229392457819No Hit
GGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAGN1653300.10389714985288967No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)