FastQCFastQC Report
Thu 6 Jul 2023
SRR1803175.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803175.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183144672
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCN26593721.4520608057874596No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAN20395021.113601601252151No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN15996450.8734324523511118Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATN12105970.6610058522477793No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAN11471180.6263452752805171No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCN9976220.5447180030440635No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACN8982500.49045925835068793No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCN7995270.4365548783204569No Hit
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACAN7838600.4280004389098472No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCN6067990.331322223777277No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTN6031280.3293177974623253Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACN5328720.29095686714817454No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGN4830130.2637330339590769No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGN4147090.2264379277165104No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGN3920240.21405154499935442No Hit
CGTAGACGACCTGCTTAGATCGGAAGAGCACACGTN3838590.2095933208474664No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTN3797160.20733117477750052No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGN3592690.19616677683094158No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGN3513600.19184833288516306Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGACTCTTAGCGGTGGATCACTCGGCTAGATCGGAN3204410.1749660508824412No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTN3153240.17217208480954335No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCN2984620.1629651557649463No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGN2971210.16223294773216226No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGAGATCGN2849660.15559611802411594No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAN2741430.1496865822009826No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGN2560670.1398167892102261No Hit
GCGCTAAACCATTCGTAGACGACCTGCTTAGATCGN2317900.12656114833632723No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGN2260510.12342756004389797No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAN2170930.11853634486292891No Hit
TACGCCTGTCTGAGCGTCGCGAGATCGGAAGAGCAN2132300.11642708339339514No Hit
GCCGCCGGTGAAATACCACTACTCTGAGATCGGAAN2083020.11373631442578903No Hit
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAN1999750.10918963561222246No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTN1996200.10899579977953168Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAN1980080.10811562129418649No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCAN1956960.10685323130776088No Hit
ACTACTCTGAGATCGGAAGAGCACACGTCTGAACTN1916780.10465933729155931Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCN1897430.10360279549928703No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)