FastQCFastQC Report
Fri 30 Jun 2023
SRR1803177.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803177.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149423011
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATN17467651.1690066933532748TruSeq Adapter, Index 1 (97% over 48bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCN8953800.5992249747932065Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGN7498020.501798213663356No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN6130870.41030293520186123Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN5851730.3916217429188333TruSeq Adapter, Index 1 (97% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGN5441160.36414471663939363No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAN5399400.3613499663716454Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGN4676940.31299998365044324No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACN4492940.3006859499036597No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACN4231130.28316455221210873No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCN3259930.21816786973995594No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN3257220.21798650543857667Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTN3002510.20094026883182No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTN2920420.1954464697542469Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CGAACTGTAGAGTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN2887880.19326875965576681Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGTCTGTAGCTCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACN2803880.18764713555397436Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTN2784930.18637892392624855No Hit
GGGTCTGTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN2622620.17551647383146363TruSeq Adapter, Index 1 (97% over 37bp)
GGGTCTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCN2201060.14730395173203945TruSeq Adapter, Index 1 (97% over 36bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCN2167360.1450486096816775No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTN2101640.14065035806298937No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTN2027440.13568459010640604Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCN1862500.12464612963795783Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAN1789580.11976602452483037Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATN1754560.11742234266715451TruSeq Adapter, Index 1 (97% over 35bp)
CTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAN1733980.11604504476221537Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCGGCTGTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAN1714780.11476010211037709TruSeq Adapter, Index 1 (97% over 37bp)
GGGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATN1626650.10886208149024651TruSeq Adapter, Index 1 (97% over 41bp)
TACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCN1616780.1082015406582859Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGN1572930.10526691902895734TruSeq Adapter, Index 1 (97% over 39bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACN1536050.10279875835188465No Hit
GTGCCGAAGCTGCGGATTTGACAGATCGGAAGAGCACACGTCTGAACTCN1502620.10056148580756413Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACN1496340.1001412024818587No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)