FastQCFastQC Report
Fri 30 Jun 2023
SRR1803183.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803183.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121795163
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT62157835.103472787338854No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT61185145.023610009865498TruSeq Adapter, Index 1 (100% over 35bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG34571302.8384788975568758No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC32063452.632571705659608No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG31205502.5621296635565076No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATC25373452.08328880843979No Hit
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGG8613950.707248940583954No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATC8025150.6589054772232621No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATC7831390.6429967994705996No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG6839830.561584699385804No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGG6723730.552052301124635No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGA6394760.525042197283319Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGA6250350.5131854045796548TruSeq Adapter, Index 1 (100% over 36bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCG6090970.500099499025261No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG5807560.47683010202958553No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC5467670.4489234108582785No Hit
ATTGATCATCGACACTTCGAACGCACTAGATCGGAA4739930.38917226950958633No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGA4599700.3776586759853509No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT4467830.36683148081997313Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG4398490.3611383154846634No Hit
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA4354490.35752569254330735Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGCTAATACATGCCGACGGGCGCTGACCAGATCG4290870.35230216819037385No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG4163030.34180585644439754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGAT3988900.32750890115398096No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGAT3838200.31513566757983646No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA3692810.3031984119106602No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCG3335950.27389839775492564No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCG3256050.2673382029136904No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGAT2988020.2453315818461526No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCG2889700.2372590116735588No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATC2810110.23072426940304683No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGA2774130.22777012909781977No Hit
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGA2669010.21913924447065275No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATC2660050.21840358307168567No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGGTAGATCGGA2433050.1997657328969624No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA2399810.1970365604749016No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT2370670.19464401882692173Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCTAATACATGCCGACGGGCGCTGACAGATCGG2370200.1946054294455027No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCG2288580.1879040138892872No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAAAGATCGG2227860.18291859423021584No Hit
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAA2212890.18168948137948632Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCG2197150.18039714762728304No Hit
TGGCGGCGGCGGCGGCGGCGGCGGGACAGATCGGAA2171000.17825010012918163No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAG2093170.17185986277632387No Hit
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGA2070810.1700239934815802No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC2032030.16683995898917595No Hit
CGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATC1965420.161370940486364No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC1932660.1586811784963907No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGATCGGA1913890.15714006639163497No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGAGATCGG1871490.15365881155723732No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC1819310.14937456916905642No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGG1792700.1471897533402045No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT1774110.14566342014748157No Hit
ATACCACCCTGAACGCGCCCGATCTCGTAGATCGGA1703250.13984545511056135No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAG1674740.1375046396546963No Hit
ATTAATGATGAGATATAACCTTGACTGAAGAGATCG1673910.13743649244921163No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAGATC1668670.13700626189892287No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCG1665550.136750094090354No Hit
GCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTG1647500.13526809763372952Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCAC1637820.13447332058663117No Hit
GCGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGA1610750.13225073642702873Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGG1551680.12740079012825822No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGA1549790.1272456115519136No Hit
GCGCTAAACCATTCGTAGACGACCTGCTTAGATCGG1524030.12513058502988333No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGA1465300.12030855445384148No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACAGATCG1451460.11917222032865131No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGA1414250.11611709079119997No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA1388210.11397907485045197No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTAGATCGGAA1329530.10916114952775259No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGAT1316360.10807982579735126No Hit
AGTCGGCATTGGCAATTTTTGACAGTCTCTAGATCG1312170.10773580556725393No Hit
TGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAAC1278790.10499513843583426Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGCCGCCGGTGAAATACCACTACTCTGAGATCGG1264700.10383827804393185No Hit
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGA1262430.10365189954218461Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC1250770.10269455446272527No Hit
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCG1245930.1022971659391761No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)