FastQCFastQC Report
Mon 3 Jul 2023
SRR1803186.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803186.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97598430
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAG1619806316.596642999277755No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGAT57035645.843909579283191No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATC29631093.0360211737012572No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGA22375982.292657781482755Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCG20682372.119129375339337No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGA19411651.9889305596411746No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCG18695561.9155595023403555No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT18318251.8769000689867654TruSeq Adapter, Index 1 (100% over 35bp)
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCAC11166801.1441577492588764No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCG10886521.1154400741897181No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCA9696770.9935374985027936No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGAT5210720.533893834152865No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCA4965610.50877970065707No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAA4193930.4297128550121145Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGC3856880.39517848801461253No Hit
GCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTG3668300.3758564558876613Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGA3541430.36285727137209073No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGA3517880.36044432272117494No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATC3499780.3585897846922333No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAGA3481570.35672397599018757No Hit
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTC3257540.3337697133037898No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCG3234240.33138237981901963No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAG3133170.32102668044967525No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACA2940650.3013009533042693No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGG2737900.28052705356018537No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG2532980.2595308141739575No Hit
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2510350.25721212933445753No Hit
GTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAG2425300.24849784981172343No Hit
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC2319770.23768517587834148Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATA2285110.2341338892439151No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGA2142390.21951070319471327No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA2016650.20662729923012083TruSeq Adapter, Index 3 (100% over 36bp)
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGC2014800.20643774700064335No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCG1979050.20277477824182213No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCA1957280.200544209573863No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC1951140.19991510109332702No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGA1950220.1998208372819112No Hit
CGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCT1902040.19488428246233058Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCAGAT1789570.18336053151674672No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACAGATCGG1718560.17608479972474966No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCG1702550.17444440448478526No Hit
CGACCGCTCTAGATCGGAAGAGCACACGTCTGAACT1684490.1725939648824269Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT1625400.1665395642122522Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA1548300.15863984697294822Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGAGATCGG1523130.15606091204540892No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA1514250.1551510613439171No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG1512540.1549758536074812Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGAT1490460.15271352213350153No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGG1432410.14676568055449252No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGATCGG1387260.14213958154859663No Hit
GGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAA1381070.1415053500348315No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGA1376860.14107399063693957No Hit
CTGGGTCGGGGTTTCGTACGTAAGATCGGAAGAGCA1365780.13993872647336641No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC1275320.13067013475524145No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT1221810.1251874645934366No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1212210.12420384221344544No Hit
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGA1210500.12402863447700953No Hit
TCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGG1131480.11593219276170733No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG1124350.11520164822323474No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGA1104300.11314731189835739TruSeq Adapter, Index 1 (100% over 36bp)
AGTCGGCATTGGCAATTTTTGACAGTCTCTAGATCG1101800.11289116023690134No Hit
AATACATGCCGACGGGCGCTGACCCCCTTAGATCGG1046080.10718205200636938No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCG1018770.1043838512566237No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGG1008530.1033346540512998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)