FastQCFastQC Report
Fri 30 Jun 2023
SRR1803194.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803194.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17660537
Total Bases883 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG12012416.801837339374221No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC10853126.14540769626654TruSeq Adapter, Index 1 (100% over 36bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA10538615.967321378732708No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG6573073.7218970181937276TruSeq Adapter, Index 1 (100% over 49bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT4936592.7952660782625127No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC3546302.008036335474963No Hit
GCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT3110171.7610846148109767TruSeq Adapter, Index 1 (100% over 35bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGAGATCGGA2980951.6879158317779352No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC2656031.5039350162455423No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG1997831.1312396672875802TruSeq Adapter, Index 1 (97% over 49bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC1905051.0787044584204886No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAA1804091.0215374538158155No Hit
CGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG1767361.0007396717325188TruSeq Adapter, Index 1 (100% over 39bp)
GCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC1742120.9864479205813504TruSeq Adapter, Index 1 (100% over 38bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG1614390.914122826502954No Hit
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC1508390.8541020015416293Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1454120.8233724716298265Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA1257660.7121301011401862Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1254460.7103181517073914No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAGATCGGAAGAGCA1099380.6225065523205778No Hit
CGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1085990.6149246764127274Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1073520.6078637359668055No Hit
GCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1056160.5980339102938942TruSeq Adapter, Index 1 (100% over 37bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG996860.5644562223674172No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG952220.5391795277799311Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA905570.5127647024549706TruSeq Adapter, Index 1 (100% over 37bp)
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGATCGGAAGAGCACA770060.43603430631809215No Hit
TTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACTC708200.40100705884537935Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC673640.3814380049711965Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGAGATCGGAA584710.331082797765436No Hit
CGACCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC581830.32945204327592076TruSeq Adapter, Index 1 (100% over 38bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGAA549810.3113212242640187No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG519480.2941473410463113No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGAGATCGGAAG511980.2899005845631987No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT496560.2811692532339192TruSeq Adapter, Index 1 (100% over 48bp)
GGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT478460.27092041425467417TruSeq Adapter, Index 1 (100% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT466530.2641652402755364No Hit
AGTCGGCACTGGCAATTTTTGACAGTCTCTAGATCGGAAGAGCACACGTC462550.26191162816849795No Hit
GCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA452270.25609074061564496TruSeq Adapter, Index 1 (100% over 37bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA438540.2483163450805601TruSeq Adapter, Index 1 (100% over 47bp)
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACAC434090.24579660290057997No Hit
ACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT421180.23848651940764884TruSeq Adapter, Index 1 (100% over 41bp)
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA419320.2374333237998369Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT411960.23326584010440907No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCAC410110.2322183068385746No Hit
GCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC407090.2305082795613746TruSeq Adapter, Index 1 (100% over 44bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACAC404010.2287642782323097No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG380200.21528224198392154No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGA379200.21471600778617322No Hit
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT371040.21009553673254666TruSeq Adapter, Index 1 (100% over 35bp)
TCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC369810.20939906866931624TruSeq Adapter, Index 1 (100% over 36bp)
ACGGCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA353210.19999958098669365Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA347240.19661916282613603Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA338390.19160799017606317No Hit
TCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT331580.18775193528939693TruSeq Adapter, Index 1 (100% over 41bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCAC323870.18338626962475715No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC311520.1763932772825651No Hit
CGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC298340.16893031055624186TruSeq Adapter, Index 1 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG290510.1644966967878723No Hit
CGGCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC284560.16112760331126963Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT278990.15797367882981134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTC275620.15606546958339942Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA267820.1516488428409623TruSeq Adapter, Index 1 (100% over 40bp)
GCGCGACCGCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC266890.15112224503705635TruSeq Adapter, Index 1 (100% over 36bp)
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACG242760.13745901384538875No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG234790.13294612728933441Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCGGCATTGGCAATTTTTGACAGTCTCTAGATCGGAAGAGCACACGTC222980.12625890141392643No Hit
CTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA213790.12105520913661913TruSeq Adapter, Index 1 (100% over 37bp)
GTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTGAACT206350.11684242670537141Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG200480.11351863196458863TruSeq Adapter, Index 1 (100% over 39bp)
GCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC193990.10984377202120185TruSeq Adapter, Index 1 (100% over 38bp)
CGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACACGTCTG192790.10916429098390384Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAA192410.10894912198875946No Hit
CGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC187750.10631047062725216TruSeq Adapter, Index 1 (100% over 42bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCA185560.10507041773418328No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG183910.1041361313078985No Hit
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC183590.10395493636461903Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACACG182320.10323581893347863No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG182110.10311690975195148No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)