FastQCFastQC Report
Fri 30 Jun 2023
SRR1803195.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1803195.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10658437
Total Bases532.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG3192402.9951858795055974No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTC2982072.79784925313158No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2395622.2476278651363235No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1934311.8148158121120386No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG1785931.675602154424706No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT1532111.4374621719863803Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1437761.3489407499429795No Hit
AAATGGATTTTTGGAAATAGGAGATGGAATAGATCGGAAGAGCACACGTC1064110.998373401278255No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1045440.9808567616433816TruSeq Adapter, Index 1 (100% over 49bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC893210.8380309420602664No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT887040.832242100788324No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG821500.7707509084118056No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA775390.7274894058106268No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT680370.6383393737749727Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC676010.6342487177059826No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACG554530.5202732820956768No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA546920.5131333984523246Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC446300.41872931275007774No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG441110.4138599308697889No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACGTC410900.38551618778625796No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT383370.3596868846717394No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT354830.332909975449496No Hit
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC343880.32263642408356874TruSeq Adapter, Index 1 (100% over 36bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT310690.29149677387031514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC297210.27884951611573067No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA293580.2754437634711356Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC284900.2672999802879165No Hit
CGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG256380.2405418355430538Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACGT239300.22451697185994535No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG238170.22345677888793639Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG222270.20853901937028851Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG218700.20518956015783554No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG215370.20206527467395077No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG210220.19723342174842334TruSeq Adapter, Index 1 (97% over 49bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCAGATCGGAAGAGCACAC210050.19707392369068746No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAACAGATCGGAAGAGCACACGTC194100.1821092529795879No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG191200.17938840375938797No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG184320.17293342354043093No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG180440.16929311492857724No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT180390.16924620373512553No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA180000.1688802964262021No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG179440.1683548910595428No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT175540.1646958179703084No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC170180.1596669380322837No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG168260.15786554820373758Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCA163140.15306184199428116Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTAATGATGAGATATAACCTTGACTGAAGAGATCGGAAGAGCACACGTC161200.1512416876883543No Hit
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC157500.14777025937292682Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT153200.14373589673607867Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA152060.1426663215253794Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGA149130.1399173255891084Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA146600.13754361920045124Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC146240.13720585860759885No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG144390.1354701444498851Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACGTCTG143120.13427860013621135Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT143020.1341847777493079No Hit
TCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG142540.13373443029217136Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC140540.13185798255410244No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT138940.13035682436364732Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA138750.13017856182853077No Hit
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT133160.12493389040062815Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTAGATCGGAAGAGCACACG128300.1203741223971207No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT127910.12000821508819726Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC121810.11428504948708708Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGAGGTGATGAACTGTCTGAGCCTGACCAGATCGGAAGAGCACACGTCT121080.11360014606269193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT119160.11179875623414577TruSeq Adapter, Index 1 (100% over 35bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCAGATCGGAAGAGCACACG118680.11134840877700923No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA117540.11027883356630995Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC117070.10983786834786377No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCGAGATCGGAAGAGCACAC114300.10723898823063831No Hit
CGTGGGGGGCCTAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG113890.10685431644433419No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG112280.10534377601518871Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC111100.10423667184972807Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGGAGCAGGGAGATGGAATAGATCGGAAGAGCACACGTCTGAACTCCA110840.1039927336437791Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG110320.10350485723188119No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGATAGATCGGAAGAGCACACGT109340.10258539784022741No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGT108280.10159088053905088No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)