FastQCFastQC Report
Sat 30 Sept 2023
SRR18054549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18054549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161870989
Total Bases8.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCCGAAGAAGAGCGCTAAGAAAACACCAGATCGGAAGAGCACACG52789033.261179185110187No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA35003132.162409102226465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA28377531.753095485195312Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT26640381.6457785403411602Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC21432941.324075433924729No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA14232590.8792551455900476No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT11373160.7026064441973601Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG10294220.6359521285188416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG9131940.5641492682793209Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT9131560.5641257927941615No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA8666620.5354029189257625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT8538960.527516391464069No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG4593080.283749424673003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT4448560.27482132700134426No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC4199630.25944303089418946No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG3343670.2065638827968117No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC2828040.17470950276333952No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC2758710.17042646227360728No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC2726500.16843660601838914No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTAGATCGGAAGAGC2605790.16097943282474167No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT2604670.1609102419211141Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA2557510.1579968106576528No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG2166550.13384424308422554TruSeq Adapter, Index 1 (100% over 49bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1965320.12141273814049533No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCAGATCGGAAGAGCACACGT1813010.11200339302306975No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCAGATCGGAAGAGCACACGTCT1698620.10493665421417793Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG1636590.10110459014987547No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)