FastQCFastQC Report
Fri 30 Jun 2023
SRR1810487.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1810487.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17486318
Total Bases891.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG4059112.321306292153671No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT3187731.822985261963096Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC2855691.6330996611179094Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC2816171.610499134237408No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC2757951.5772045321376402No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT2511261.4361285205953593No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG2502431.4310788583394172No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC2327561.3310749581472783No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA2170911.2414906328479214Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT1899531.0862950107621285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1761391.0072961043028041No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT1649910.9435434034769355No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1432050.818954567794089No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1392470.7963197283727769Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1346330.7699333844895192No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG1302720.7449938860771033No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC1246700.7129574104737201No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1199960.6862279411823575No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1199420.6859191283150632No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG1187850.6793025266954427No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT1118370.6395686044369089No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1102420.6304471873381234Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1016800.581483191601571No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA1000480.5721501805011209No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT891290.5097070749828524Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC875040.5004140951800145No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC835290.47768203689307265No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC824650.4715972796560145Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG776470.44404430938520045Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC760950.4351687988288901No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACG745710.4264534134630287No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG744240.4256127562131719Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG672280.38446058226780505No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA671570.384054550534881No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA645950.36940309560880685No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA640120.3660690603933887No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA616410.35250988801644806Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC599860.3430453455095578No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG569040.32542013704657546No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC523850.2995770750594836No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC512380.29301766100788057No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA509480.2913592215353741No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT502330.2872703104221255No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT498800.285251589271109No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC481160.2751637022728284Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC463340.26497287765211636Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCAGATCGGAAGAGCACAC459630.26285121887866847No Hit
ATGGTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAA403710.23087193084330274Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAACGCGCCCGATCTCGTCTGATCTCGGAAGCAGATCGGAAGAGCACACG402260.23004271110704952No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC385860.22066394995218547No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC383300.2191999482109384No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA381690.21827922836585725No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGT378670.21655216381172981No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT378640.2165350075413246No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG344540.19703404684736947No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC339540.19417466844649628No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG333900.19094928961031132TruSeq Adapter, Index 4 (100% over 49bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC324010.18529343913338417Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC319470.18269712354539133No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA309020.17672102268756637No Hit
AGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGTC288920.16522632151605615No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTC273890.15663103004303136No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACAC273810.1565852799886174No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT264660.15135261751501947No Hit
ATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG262690.15022602242507543Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT257960.14752105045784938No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC256460.14666323693758743No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG252780.14455873443454476Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG241920.1383481645478482No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC220840.12629302520976687No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACGT218480.12494339860455472No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG217720.124508773087622No Hit
AACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGA214490.12266161464065792Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAAC212240.12137489436026497Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA207560.11869851617704767Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC204960.11721163940859361No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA201540.11525582458239636Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAGTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAAC189490.10836472263629199Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT183350.10485340596001973No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC182820.10455031184952715No Hit
TTAGTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACT182310.10425865525263808Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC182100.10413856135980142No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTC180520.10323499778512549No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC180130.10301196626985738No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC179400.1025944970233299Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC178580.10212555896558669Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)