FastQCFastQC Report
Fri 30 Jun 2023
SRR1810488.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1810488.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22770301
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC6775062.975393254573139No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC5497722.4144257030243037Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG4862762.1355712425584534No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT4798722.1074468888224183Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA4661632.0472412727438254No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT3351281.4717767674656563Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT3342631.467977959535976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG3307121.452383084439683Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA2770891.216887734597799Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG2707481.1890400570462376No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC2306801.0130740037208994No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA1401740.6156001187687418Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT1281090.5626144335992748No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1102980.4843941237316099No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG1003200.440573886133521No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG994180.43661258584153106No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA968010.4251195449721986No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC964080.42339361258333824No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC948000.4163317823510546No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT931060.40889226716853677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC913120.4010135834392352No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA897010.39393857815054795No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC833810.366183125993811No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC825240.3624194515478737No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT759220.3334255440892064No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT727650.3195609930672414No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC724860.3183357128217146No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA709210.31146272506454786No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG702290.3084236787208039No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT683350.3001058264447185No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA677090.2973566313418518No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG620970.2727104924963442No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC613070.26924106097675216No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT605980.2661273559800549Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC605400.2658726382229203No Hit
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA604190.26534124428131184Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC601660.26423014785794885Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT595110.26135359387651486No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGT579560.25452452297402656No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC533820.23443695364413494No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC524850.2304976117794842No Hit
AGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGTC524270.23024289402234954No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC485140.21305822878669894No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC482870.21206131618549973No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC465560.20445930864067188Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA454680.19968115485166404No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG449650.19747213706134145No Hit
GAACGCGCCCGATCTCGTCTGATCTCGGAAGCAGATCGGAAGAGCACACG446170.19594383051853376No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT419780.18435417256890896No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC418630.18384912874010756No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACGT413100.18142052667639308No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT402530.1767785151368882No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC400050.17568937714086433No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC400020.1756762020844608No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC397440.17454314723375858No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACG380170.16695870643080213No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC363710.15972999215074057No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGT339710.14918994702792907No Hit
TCGCTGGTTCGAATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAA327880.14399458311947655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA326840.14353784783082138Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTC326070.14319968804979785No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG318090.13969512304646303Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA311210.13667364344459038Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC311040.13659898479163715No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC308610.1355318052229525Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC303420.1332525204651445Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT299570.13156172156002682Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC291500.12801763138748146No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC286330.1257471300006091No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG279630.12280470073715759TruSeq Adapter, Index 6 (100% over 49bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA278240.12219425645712807No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG277550.12189123015984725No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG274370.12049467418107473Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT268770.11803533031908538Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG268020.11770595390899752Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC267640.11753906986121966No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT256910.11282679135422935Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC249170.10942762680212265No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG241890.10623047978153648No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT233710.1026380810688449No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA233430.10251511387574543No Hit
AACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGA233260.10244045522279219Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)