FastQCFastQC Report
Tue 19 Sept 2023
SRR18113790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18113790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16368535
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA4594562.8069463760806936No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA3269981.99772307051303No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA3199991.9549642041880964No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA2113821.2913922962562012No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA1711121.0453715008704199No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA1668641.0194192699591014No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1579160.9647534125686873No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA1471760.8991397214228395No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA1403880.8576699136483503No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA1068750.6529295382879409No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA1030020.6292682882127203No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA965520.589863417831834No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT813080.49673351952389144No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA810980.4954505702556765No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA685540.41881573396763977No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA665190.4063833446304144No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAACAAAAA565580.3455287843414209No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA562040.3433660984321444No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAC536240.3276041502797899No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA502060.30672262361903496No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA497490.3039306816401101No Hit
CCCCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAA489710.2991776600654854No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA454290.27753858240826074No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAA435250.2659065090431123No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAA427560.26120847100855393No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA415510.2538467859218922No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAAAAAAAAACAAAAAA404580.24716934044494515No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA402150.2456847848631536No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAAAAAAAAAACAAAAAAAAAA372780.22774182295483375No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGCAAAAAAAAAACAAAAAAA364550.22271388367987727No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCAAAAAAAAAACAAAA352870.2155782420357106No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA332890.20337189614098022No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC331260.20237608313755628TruSeq Adapter, Index 5 (100% over 50bp)
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA331230.2023577552908675No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA329740.2014474722386579No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA320150.1955886705804765No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA304890.1862659058981149No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA296660.18123796662315841No Hit
GGGGGCGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAAA286970.17531807214268108No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA278810.17033289784333172No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAA274310.16758372084001408No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA269030.16435801982278805No Hit
CCCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAC268470.16401590001793076No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAAAAAAAAAACAAA268200.16385094939773168No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA259130.15830983041548924No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA257400.15725292458976933No Hit
GGGTGGATAACTGTGGTAATTCTAGAGCTAATACATGAAAAAAAAAACAA251670.15375230587221153No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAACAAAAAA244470.1493536226669033No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA242380.148076782680918No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCCAAAAAAAAAACAAAAA241930.14780186498058623No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC237640.14518098290409007No Hit
GGGGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAAAAAA223280.13640805362239197No Hit
GGGGGGCCGATCGCACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA217580.13292576275152296No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA215420.13160615778993048No Hit
GGGAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAC213740.13057979837535857No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA212490.12981613809665923No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAAAA210390.12853318882844433No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAACAAAAAA209630.12806888337899514No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAAAAAAAACAAAAAAAAAAGA202800.1238962436161819No Hit
GGGGGCGGAGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAAAA202310.12359688878693176No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAACAAAAAA201710.12323033185315606No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAA198520.12128147082191534No Hit
GGGGGAATAATGGAATAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA194620.11889885075237337No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTAAAAAAAAAACAAAAAAAAA188130.11493392658536639No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAACAAAAAAA186400.11387702075964648No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGCCGTC185750.11347991741472283TruSeq Adapter, Index 5 (98% over 50bp)
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA181800.11106675093403288No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA181420.11083459820930829No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGAAAAAAAAAACAAA180890.11051080625113976No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA179890.10989987802818028No Hit
GGGCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAA177840.10864747517111337No Hit
CCCCCCCTTGTAAATTTAACTGTTAGTCCAAAAAAAAAACAAAAAAAAAA173780.10616710658589788No Hit
GGGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAAAAAAAAACAAAAAA172300.10526293281591786No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATAAAAAAAAAACAAAAAAA171820.10496968726889731No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA170860.10438319617485621No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAACAA170060.10389445359648863No Hit
GGGCACTCGCCGAATCCCGGGGCCGAGGGAGCAAAAAAAAAACAAAAAAA164650.10058933191027786No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)