FastQCFastQC Report
Thu 21 Sept 2023
SRR18113793_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18113793_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73800009
Total Bases11 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACGGCGGAGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAA3611270.48933191864515896No Hit
GGGGGGGACGGCGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACA3430700.46486444195420085No Hit
CCCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAG2421500.3281164911511054No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC2279060.30881568049673275RNA PCR Primer, Index 1 (100% over 50bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA2246290.3043753016344483No Hit
GGGGGGACGGCGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAA1954130.26478723058150305No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA1898510.2572506461347451No Hit
GGGGGGACGGCGGAGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAA1864410.2526300504922703No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG1751500.23733059436347767No Hit
CCCCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG1531110.20746745437388767No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA1408300.190826534994054No Hit
CCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA1320740.17896203779595746No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCGCGTATGC1275270.17280079193486278RNA PCR Primer, Index 1 (98% over 50bp)
GGGGGGGACGGCGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAA1172130.15882518388310765No Hit
GGGCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG1130280.15315445286734317No Hit
CCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGA970510.13150540401695615No Hit
CCCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC942130.12765987603063841No Hit
GCCATGACACGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC915360.12403250519928799RNA PCR Primer, Index 1 (100% over 38bp)
CCCCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAA911110.12345662451070975No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA900620.12203521546996017No Hit
GGGGGCGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAAA837120.1134308804759089No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAACAAAAA814310.11034009494497486No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA755740.10240378154967433No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA754910.10229131543872848No Hit
GGGGGCGGAGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAAAA747980.10135229116299972No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAAAAAAAAAACAAA741580.10048508259667013No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)