FastQCFastQC Report
Tue 19 Sept 2023
SRR18113795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18113795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16683577
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA5364493.215431558831778No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA4112872.465220737735079No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA3182891.9077983096790336No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA2373081.4224047996421871No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA1705851.022472578872025No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA1376650.8251527834828227No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA961570.5763572164410545No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAACAAAAAAAAAA951380.570249413540034No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA818050.49033249884002694No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA754210.45206732345227885No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA734070.43999557169304876No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTAAAAAAAAAACAAAAAAAAA620320.3718147493190459No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA607310.3640166614149951No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA602560.36116955015102575No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA590280.3538090182938587No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA557320.33405306308113664No Hit
GGGGGCGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAAA549830.32956361816174073No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA547500.3281670351627831No Hit
CCCCCCCTTGTAAATTTAACTGTTAATCCAAAAAAAAAACAAAAAAAAAA535850.32118412016799514No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA524380.3143090957053155No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAAAAAAAACAAAAAAAAAAGAT508060.30452702079416183No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAA503180.30160198859033643No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAAAAAAAAACAAAAAA464620.2784894390453558No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAAAA452010.2709311078793235No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAAAAAAAACAAAAAAAAAAGA437930.26249167070107327No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGCAAAAAAAAAACAAAAAAA435820.2612269539080258No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA433080.2595846202525993No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAA423030.2535607322098852No Hit
GGGGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAAAAAA412570.2472910935107022No Hit
GGGGGCGGAGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAAAA411780.24681757395311568No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA386670.2317668447240061No Hit
GGGGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAAAAAAGAT382370.22918945979030755No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAAAAAAAACAAAAAAAAAAGATC358950.21515170277932602No Hit
CCCCTAAACCATTCGTAGACGACCTGCTCAAAAAAAAAACAAAAAAAAAA358020.2145942683634331No Hit
CCCCGGCGGGGCGCGGGACATGTGGCGTACAAAAAAAAAACAAAAAAAAA356140.21346741169474626No Hit
GGGGGAGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAAAAAAG326590.19575538267363168No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC305880.18334197756272533No Hit
GGGGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAAAAAAGATC293920.176173251095973No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAACAAAAAA273530.1639516513754814No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA266810.15992373817677108No Hit
GGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAACAAAAAAAAAAGATC265800.15931835241327444No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCAAAAAAAAAACAAAA264610.1586050761176695No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA255340.15304871371409143No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA242520.1453645102605994No Hit
CCCCAACTTCTTAGAGGGACAAGTGGCGTAAAAAAAAAACAAAAAAAAAA241900.14499288731667076No Hit
CCCCGGCGGGGCGCGGGACATGTGGCGTACGAAAAAAAAAACAAAAAAAA236300.14163629298441216No Hit
CCCCGCCGCGACCGGCTCCGGGACGGCTGGGAAAAAAAAAACAAAAAAAA235150.14094699236260905No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA234470.14053940590797764No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA233760.14011383769799488No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCAAAAAAAAAACAAAAAAAAAA225670.13526475767157128No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTGAAAAAAAAAACAAAAAAAA221230.13260345787956623No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCCAAAAAAAAAACAAAAAAAAA219990.13186021199170897No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAAA208110.12473943687256037No Hit
CCCCCCGCCACGCAGTTTTATCCGGCAAAAAAAAAACAAAAAAAAAAGAT205200.12299520660347597No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAAA200410.12012411966570478No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAA199170.11938087377784752No Hit
GGGGGCATGCTCATAAGGAAAGGTTAAAAAAAAAACAAAAAAAAAAGATC197980.11866759748224255No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA187430.11234401351700538No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAACAAAAAAA186080.11153483452619303No Hit
CCCCCCCGCCACGCAGTTTTATCCGGCAAAAAAAAAACAAAAAAAAAAGA183910.11023415422244283No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAACAAAAAA183090.10974265290950495No Hit
CCCCAACTTCTTAGAGGGACAAGTGGCAAAAAAAAAACAAAAAAAAAAGA182970.10967072588809942No Hit
CCCCGGCGGGGCGCGGGACATGTGGCGTACGGAAAAAAAAAACAAAAAAA181810.10897543134784585No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA181630.10886754081573755No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAA181370.10871169893602553No Hit
GGGGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAAAAAAAAACAAAA181110.10855585705631353No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAGAAAAAAAAAAC179640.10767475104409563No Hit
GGGGGGGGATGCGTGCATTTATCAAAAAAAAAACAAAAAAAAAAGATCGG178020.10670373625512083No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGAAAAAAAAAACAAAAAAAAAA176230.10563082485248816No Hit
CCCCTAAACCATTCGTAGACGACCTGCAAAAAAAAAACAAAAAAAAAAGA174240.10443803508084627No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAAAAAAAAAACAAA170130.10197453459770647No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAGAAAAAAAA169360.10151300287702092No Hit
GGGGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCT169270.10145905761096677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCTGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC167010.10010443204116239No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)