FastQCFastQC Report
Tue 19 Sept 2023
SRR18113796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18113796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14586779
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA4558473.1250696264062134No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA3602782.469894141811568No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA2833781.942704417472836No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA2079961.4259213771594126No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA1438660.9862766824670478No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA1181850.8102199944209753No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA851540.5837752117859604No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAACAAAAAAAAAA826730.5667666590410398No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA728230.49923975676878357No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA649430.44521823495097856No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA639510.43841755606223964No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA555010.3804883860926391No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTAAAAAAAAAACAAAAAAAAA554010.37980283378530655No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA517980.35510238415211476No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA516630.3541768885372158No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA504520.3458748500954186No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA485520.3328493562561001No Hit
GGGGGCGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAAA484620.33223235917950084No Hit
CCCCCCCTTGTAAATTTAACTGTTAATCCAAAAAAAAAACAAAAAAAAAA475770.3261652212596078No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA466850.3200500946782014No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAA457040.3133248265432691No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAAAAAAAACAAAAAAAAAAGAT451410.3094651670529868No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAAAAAAAAACAAAAAA412630.2828794485746305No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAAAA392500.2690792806280263No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAA391330.26827718442844717No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAAAAAAAACAAAAAAAAAAGA387760.26582976269126996No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGCAAAAAAAAAACAAAAAAA387180.2654321423530171No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA372910.2556493109273816No Hit
GGGGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAAAAAA370290.2538531638821703No Hit
GGGGGCGGAGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAAAA363000.248855487561716No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA339330.23262846444715451No Hit
GGGGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAAAAAAGAT338330.23194291213982196No Hit
CCCCTAAACCATTCGTAGACGACCTGCTCAAAAAAAAAACAAAAAAAAAA319630.21912308399270325No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAAAAAAAACAAAAAAAAAAGATC318820.2185677866237639No Hit
CCCCGGCGGGGCGCGGGACATGTGGCGTACAAAAAAAAAACAAAAAAAAA301550.20672829827613073No Hit
GGGGGAGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAAAAAAG292860.20077084872541084No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC269280.18460552531850932No Hit
GGGGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAAAAAAGATC261240.1790936847675556No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA246080.16870071178839413No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAACAAAAAA244760.16779578274271517No Hit
GGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAACAAAAAAAAAAGATC239360.16409380028311937No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCAAAAAAAAAACAAAA224630.15399561479611093No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA217820.14932700358317624No Hit
CCCCAACTTCTTAGAGGGACAAGTGGCGTAAAAAAAAAACAAAAAAAAAA212590.145741565015827No Hit
CCCCGGCGGGGCGCGGGACATGTGGCGTACGAAAAAAAAAACAAAAAAAA210070.14401397320134898No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA208260.14277312352507704No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA202340.13871465386566836No Hit
CCCCGCCGCGACCGGCTCCGGGACGGCTGGGAAAAAAAAAACAAAAAAAA201820.13835816666585543No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA199050.13645918677454427No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAA198460.13605471091321805No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTGAAAAAAAAAACAAAAAAAA197510.13540343622125214No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCAAAAAAAAAACAAAAAAAAAA194780.13353187842223427No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCCAAAAAAAAAACAAAAAAAAA190110.13033034914699126No Hit
CCCCCCGCCACGCAGTTTTATCCGGCAAAAAAAAAACAAAAAAAAAAGAT185610.12724536376399478No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAAA181860.12467454261149771No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAAA180830.1239684237349452No Hit
GGGGGCATGCTCATAAGGAAAGGTTAAAAAAAAAACAAAAAAAAAAGATC176490.1209931267211219No Hit
CCCCCCCGCCACGCAGTTTTATCCGGCAAAAAAAAAACAAAAAAAAAAGA165750.11363029494037033No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA165100.11318468594060414No Hit
GGGGGGGGATGCGTGCATTTATCAAAAAAAAAACAAAAAAAAAAGATCGG164770.11295845367918442No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAACAAAAAA164650.11287618740230451No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAACAAAAAAA163670.1122043461411186No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA163040.11177244818749911No Hit
CCCCGGCGGGGCGCGGGACATGTGGCGTACGGAAAAAAAAAACAAAAAAA162360.11130627261851297No Hit
GGGGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAAAAAAAAACAAAA161390.1106412868804004No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA160540.11005856741916774No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGAAAAAAAAAACAAAAAAAAAA159770.10953069214252166No Hit
CCCCAACTTCTTAGAGGGACAAGTGGCAAAAAAAAAACAAAAAAAAAAGA159260.10918106046578208No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAA157360.10787851108185022No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAGAAAAAAAAAAC156000.10694615994387795No Hit
CCCCTAAACCATTCGTAGACGACCTGCAAAAAAAAAACAAAAAAAAAAGA152040.10423137280684104No Hit
CCCCTGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC150830.10340185451496865No Hit
GGGGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCT148340.1016948292697106Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAAAAAAAAAACAAA146030.10011120343977242No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)