FastQCFastQC Report
Thu 21 Sept 2023
SRR18113799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18113799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59859326
Total Bases8.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC32253565.3882263893181825TruSeq Adapter, Index 6 (100% over 50bp)
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG16673962.7855241804760715No Hit
GGGCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG13121282.192019335466624No Hit
CCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA10555331.7633559723007908No Hit
CCCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAG8510641.4217734426211213No Hit
GGGCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA6967101.163912203087619No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAA6781151.1328477036310098No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAA6686851.1170941015941274No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAA5883070.9828159441688334No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA5544320.9262249294287076No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAA5493860.9177951652846875No Hit
GGGGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC5451240.9106751385740628No Hit
CCCCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG4372160.7304058184684539No Hit
CCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGA3872480.6469301040910485No Hit
GGGGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC3411380.5698995007060387No Hit
GGGGGGGACGGCGGAGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAA3340370.5580366875497396No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT3089920.5161969247699181No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA3089080.5160565957591972No Hit
CCCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC3069160.5127287935049586No Hit
GGGGGGGACGGCGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACA3069050.512710417086888No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA2869120.47931044195185224No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAAAAAAAAAACAAAAAAAAAA2253570.37647767701226703No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA2187650.36546519083759815No Hit
CCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC1960320.3274878170195234No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAC1455200.243103305239354No Hit
GGGGGCGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAAA1444170.2412606516819117No Hit
GGGGGGACGGCGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAA1339500.22377465459601067No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1318290.22023134707530784No Hit
GGGGGGACGGCGGAGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAA1308040.21851899902782068No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAAACAAAAAAAAAAGATCGGAAGA1240410.20722084308132704No Hit
GGGGGCGGAGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAAAA1236860.20662778595268513No Hit
CCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC1195670.19974665267697805No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA1124360.18783372201684997No Hit
GGGGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAA1052400.17581220343175932No Hit
GGGCGGGGAGGTAGTGACAAAAAAAAAAACAAAAAAAAAAGATCGGAAGA994620.16615957219431438No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC988350.1651121163642905No Hit
GGGGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAAAA912470.1524357290625023No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA891570.14894420962908939No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA874820.14614598233197612No Hit
CCCCGGGGAGGTAGTGACGAAAAAAAAAACAAAAAAAAAAGATCGGAAGA854400.14273465090468945No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAA849820.14196952367956833No Hit
GGGCGGGGAGGTAGTGACGAAAAAAAAAACAAAAAAAAAAGATCGGAAGA817630.13659191551872804No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAGATCGGAAGAGC808180.13501321414811787No Hit
GGGCGGGGAGGTAGTGACAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC789280.13185581140689756No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA764740.12775619959369405No Hit
CCCCGGGGAGGTAGTGAAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC706750.11806848610356889No Hit
CCCCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAA700550.11703272435777176No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA694660.11604875069926447No Hit
CCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAAAAAAAAAACAAA691070.11544901123677871No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAAGATCGGAAGA678620.11336913482787962No Hit
GGGCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC657760.10988429772831054No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAAAAA655460.10950006353228903No Hit
GGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGAAAAAAAAAACAAA640760.10704430584467321No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA630660.10535701654910049No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA615280.10278765918613918No Hit
CCCCCCGGGGAGGTAGTGACAAAAAAAAAAACAAAAAAAAAAGATCGGAA610980.10206930829792504No Hit
GGGGGCGGGGCGCGGGACATGTGGCGTACGGAAGAAAAAAAAAACAAAAA604890.1010519229701985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)