FastQCFastQC Report
Tue 19 Sept 2023
SRR18113802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18113802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12858478
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC1722951.3399330776161844TruSeq Adapter, Index 9 (100% over 50bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA1623531.26261444006048No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1136070.8835182515380124No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA934500.7267578635667457No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA806140.6269326743025108No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA754970.5871379178779946No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA698410.543151374525041No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGCCGTC603870.4696278984184598TruSeq Adapter, Index 9 (98% over 50bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA597620.4647672920543162No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG587780.4571147533946086No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA568650.442237409435238No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAA461150.3586349799719687No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA422110.32827368837898235No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA391900.30477946145725804No Hit
CCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA387190.30111650850123944No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT385210.29957666840507874No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAAAAAAAAAACAAA370680.28827673072971777No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA350780.27280056006628467No Hit
GGGCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG350250.2723883806466053No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA349250.27161068362834234No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA336910.2620139024229773No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA327660.25482020500404484No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAACAAAAA316100.24583002747292484No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGCAAAAAAAAAACAAAAAAA286440.22276353391124515No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA270090.21004818766264563No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA257410.20018698947107116No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA253880.19744171899660287No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAAAAAAAAAACAAAAAAAAAA251720.19576189343715483No Hit
CCCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAG244310.18999915853182622No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAAAAAAAAACAAAAAA238810.1857218249313799No Hit
GGGGGCGGAGCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAAA227400.17684830195299941No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA225970.17573619521688336No Hit
GGGCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA200660.15605268368464761No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA200520.15594380610209077No Hit
CCCCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAA198550.15441174297611274No Hit
CCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAAAAAAAAAACAAA196650.1529341186414131No Hit
GGGGGCGGAGCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAAAA196260.15263081680429053No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA195080.15171313432274022No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCAAAAAAAAAACAAA192330.14957446752251705No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA184730.14366397018371846No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA183840.14297181983746443No Hit
GGGGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAAAA183630.1428085034636292No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAAAAA181390.14106646214272017No Hit
GGGTGGATAACTGTGGTAATTCTAGAGCTAATACATGAAAAAAAAAACAA181040.14079426818632812No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA171160.13311062164588996No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA169840.13208406158178285No Hit
CCCCGGCGGGGCGCGGGACATGTGGCGTACAAAAAAAAAACAAAAAAAAA167390.1301787038870386No Hit
CCCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAA165550.1287477413734347No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA157800.12272058948189668No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA154230.1199442111266979No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA152450.1185599104341898No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCCAAAAAAAAAACAAAAA152240.11839659406035458No Hit
GGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGAAAAAAAAAACAAA151450.11778221341592683No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAACAAAAAA147060.11436812350575239No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAAA139930.10882314376553742No Hit
CCCCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG137300.10677780060750582No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA136960.10651338362129639No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAA135660.10550237749755453No Hit
GGGGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAAAAAAAAAACAAAAA134720.10477134230038734No Hit
CCCCGGGGAGGTAGTGACGAAAAATAACAATACAAAAAAAAAACAAAAAA133290.1036592355642713No Hit
CCCCGGCGGGGCGCGGGACATGTGGCGTACGGAAGAAAAAAAAAACAAAA132960.10340259554824452No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTCAAAAAAAAAACAAAAAAAAA131010.10188608636263173No Hit
CCCCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGATC130720.10166055422733546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)