FastQCFastQC Report
Tue 19 Sept 2023
SRR18113900_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18113900_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12679748
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA4845703.8216059183510587No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA3765682.9698382018317715No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA3048802.404464189666861No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA2715402.1415252101224724No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA2201031.735862573924971No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1355881.0693272453048752No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA1246190.9828192169118819No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA1229610.9697432472632738No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA1177610.9287329685100997No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA1174950.9266351350200335No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA873730.6890752087502054No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA830790.6552101824105653No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAAAAAAAAACAAAAAA829990.6545792550451318No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA745170.5876851811250507No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT715720.5644591674850321No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA713780.562929168623856No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGCAAAAAAAAAACAAAAAAA694470.5477001593407061No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA655620.5170607491568444No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAA653010.5150023486271178No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA558460.44043462062495253No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA525420.4143773204325512No Hit
GGGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAAAAAAAAACAAAAAA509960.40218464909554985No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA462220.36453405856330895No Hit
GGGCACTCGCCGAATCCCGGGGCCGAGGGAGCAAAAAAAAAACAAAAAAA429070.3383900058581606No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA419860.33112645456360806No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA360920.284642880915299No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA354360.2794692765187447No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC336060.2650368130344546TruSeq Adapter, Index 12 (100% over 50bp)
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA335550.2646345968389908No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA334740.26399578288148945No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAACAA329250.25966604383620245No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA328950.2594294460741649No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAAAAAAAAAACAAAAAAAAAA320350.2526469768957553No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGAAAAAAAAAACAAAAAAAA302580.23863250279106493No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA286340.22582467727276598No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAAACAAAAA280220.2209980829272001No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAAA275050.21692071482808647No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA271660.21424716011706227No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA271440.21407365509156806No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA254160.20044562399820562No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA253360.19981469663277218No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA248990.196368255899092No Hit
GGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA239030.18851321019944559No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAACAAAAAA238650.1882135197008647No Hit
CCCCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAA236290.18635228397283604No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAAA225730.17802404274911457No Hit
CCCCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGATC223560.17631265227037635No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA220110.1735917780069446No Hit
GGGAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAC217600.17161224339789719No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA213590.16844971997866204No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAA213520.1683945138341866No Hit
GGGGCGCCGCGACCGGCTCCGGGACGGCTGGGAAAAAAAAAACAAAAAAA206620.16295276530732314No Hit
GGGCAACTTCTTAGAGGGACAAGTGGCGTTAAAAAAAAAACAAAAAAAAA204850.16155683851130165No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTCAAAAAAAAAACAAAAAAAAA201290.15874921173512282No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA199700.15749524359632383No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA196240.1547664827408242No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAAAAAA195920.1545141117946508No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA195610.15426962744054534No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA190040.14987679565871498No Hit
GGGTGGATAACTGTGGTAATTCTAGAGCTAATACATGAAAAAAAAAACAA187640.14798401356241464No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAAA187090.14755025099867916No Hit
GGGCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAA185670.14643035492503478No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAAAAAAAAAACAAA185570.14635148900435563No Hit
GGGCACTCGCCGAATCCCGGGGCCGAGGGAGAAAAAAAAAACAAAAAAAA184710.14567324208651466No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA181270.14296025441515084No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA177090.13966365893076108No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAAA173520.13684814556251432No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAAA169220.13345691097330958No Hit
GGGGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAAA168170.1326288188061782No Hit
GGGGGAATAATGGAATAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA164210.12950572834728263No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA163790.12917449148043006No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA159890.126098720573942No Hit
CCCCAACTTCTTAGAGGGACAAGTGGCGTTAAAAAAAAAACAAAAAAAAA159080.12545990661644063No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA158420.12493939153995805No Hit
GGGGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAAAAAAAAACAAAA150410.11862223129355569No Hit
GGGGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAAAA147010.11594078999046353No Hit
GGGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAAAAAAAAACAAAAA145590.11482089391681917No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAACAA143980.11355115259388436No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAC142020.11200538054857241No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAACAAAA140820.11105898950042226No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGCCGTC139120.10971826884887619TruSeq Adapter, Index 12 (98% over 50bp)
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAAAAAAAAAACAA138110.10892172305001645No Hit
GGGTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAA136060.10730497167609325No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAA134200.10583806555146048No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA132220.1042765203220127No Hit
GGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGAAAAAAAAAACAAA131380.10361404658830758No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA130570.10297523263080623No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAAAAAAAAAACAAAAAAAAAA128740.10153198628237722No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)