FastQCFastQC Report
Tue 19 Sept 2023
SRR18113901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18113901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38395715
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA14496963.7756713216565965No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA11424742.9755247428000753No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA9321792.4278203961040963No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA8361742.1777794735688603No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA6633011.7275391277386032No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA4200331.093958010679056No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA3789950.9870762922372979No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA3707060.9654879457251936No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA3601390.9379666454967696No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA3538230.9215168932262364No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA2667560.6947546099870779No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA2573390.6702284356470507No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAAAAAAAAACAAAAAA2511180.6540261068194719No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA2251810.5864742979783031No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT2203030.5737697552969127No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA2154830.5612162711385893No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGCAAAAAAAAAACAAAAAAA2114110.5506109210363709No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAA2022140.5266577273010804No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA1988410.5178728928475482No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA1719050.44771923116941564No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1548760.40336792790549675No Hit
GGGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAAAAAAAAACAAAAAA1540900.40132082447221No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA1432540.3730989252316307No Hit
GGGCACTCGCCGAATCCCGGGGCCGAGGGAGCAAAAAAAAAACAAAAAAA1307670.3405770669982314No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA1278110.3328782912364049No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA1091220.28420358886401775No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA1053560.27439520269384227No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA1003760.2614250053684376No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA1002870.26119320866924867No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA1001280.26077909995946164No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAACAA999840.26040405810908845No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAAAAAAAAAACAAAAAAAAAA988560.2574662302811655No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC950410.24753022570357133TruSeq Adapter, Index 12 (100% over 50bp)
CCCCACTCGCCGAATCCCGGGGCCGAGGGAGAAAAAAAAAACAAAAAAAA918150.23912824647229514No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA866200.22559809082862503No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAAACAAAAA865500.22541577881802694No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAAA861670.22441827167432615No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA830150.21620902228282504No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA799060.20811176455497704No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA791820.20622613747393426No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA751590.1957484057791345No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA747900.19478736103755329No Hit
GGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA734160.19120883671524283No Hit
CCCCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAA732300.19072440765851084No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAACAAAAAA726690.18926330711643213No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAAA688030.17919447521683082No Hit
CCCCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGATC678810.176793165591525No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA663470.17279792810213326No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAA657760.17131078298711197No Hit
GGGAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAC651240.16961267683125578No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA647540.16864902763238035No Hit
GGGCAACTTCTTAGAGGGACAAGTGGCGTTAAAAAAAAAACAAAAAAAAA627750.1634948066470438No Hit
GGGGCGCCGCGACCGGCTCCGGGACGGCTGGGAAAAAAAAAACAAAAAAA626410.16314580936961326No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAAAAAA616280.16050749413052992No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTCAAAAAAAAAACAAAAAAAAA613100.1596792767109559No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA605340.1576582178506117No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA594710.1548896797468155No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA591530.15406146232724147No Hit
GGGCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAA581030.1513267821682706No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA577260.15034490176833534No Hit
GGGTGGATAACTGTGGTAATTCTAGAGCTAATACATGAAAAAAAAAACAA572710.14915987369944797No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAAA571600.14887077893978534No Hit
GGGCACTCGCCGAATCCCGGGGCCGAGGGAGAAAAAAAAAACAAAAAAAA569630.14835770085281652No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAAAAAAAAAACAAA564950.1471388148391038No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA560110.14587825750868294No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAAA530850.13825761546568413No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA530660.13820813077709324No Hit
GGGGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAAA528740.1377080749765957No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAAA518930.13515310237092862No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA494510.1287930176583507No Hit
GGGGGAATAATGGAATAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA490290.12769393668017381No Hit
CCCCAACTTCTTAGAGGGACAAGTGGCGTTAAAAAAAAAACAAAAAAAAA484660.12622762722350658No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA482320.12561818421665022No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA480750.1252092844214517No Hit
GGGGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAAAA461170.1201097570392946No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGCCGTC459570.11969304387221334TruSeq Adapter, Index 12 (98% over 50bp)
GGGGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAAAAAAAAACAAAA457200.11907578749347421No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAC441110.11488521570701313No Hit
GGGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAAAAAAAAACAAAAA436930.11379655255801331No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAACAA430170.11203593942709493No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAACAAAA429680.1119083210196763No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAAAAAAAAAACAA421150.10968671894767423No Hit
GGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGAAAAAAAAAACAAA406190.10579045083546432No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAA405520.10561595219674903No Hit
GGGTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAA404310.10530081286414382No Hit
CCCCACTCGCCGAATCCCGGGGCCGAGGGAAAAAAAAAACAAAAAAAAAA395150.10291512998260353No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA391990.10209212147761801No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA390770.10177437768771853No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAAACAAAAA389660.1014852829280559No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)