FastQCFastQC Report
Tue 19 Sept 2023
SRR18158439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18158439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26267205
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length41
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAA4916341.871664686060051No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAA2517270.9583318819036895No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAA2299850.8755594666429108No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA2207790.8405119615886044No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA1934700.7365458182551208No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGTAAAAAAAA1785870.6798858119849447No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAA1725500.6569027804823544No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAA1688310.6427444412148152No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA1546660.5888178814609321No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA1441150.5486499229742944No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAA1401180.5334332297631209No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAAAAAAAAAAA1257960.47890896652308457No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAA1254990.47777827903654No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAA1242680.47309182686167023No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGTAAAAAAAAA1238050.4713291726318045No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAAA1163160.44281833563944095No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTAAAAAAA1125530.4284924871146359No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTAAAAAAAA1108690.42208145099564265No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTCAAAAAAAAA1106010.4210611673377506No Hit
GCCGCCTGGATACCGCAGCTAGGAATAACGAAAAAAAAAAA1089820.41489758807608196No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAA1084930.4130359511032864No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAAAAAAA1044790.39775453840635117No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCCGAAAAAAAAA1032380.3930300159457392No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA1007280.3834743742244369No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAAA996090.3792143092498802No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAA989630.3767549687909315No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGAAAAAAA925660.3524014070016204No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA913590.34780632351253205No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAAA893510.34016181013549024No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTAAAAAAAAA878280.33436370561694706No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAA818360.31155199040019677No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAA798180.3038694067374127No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCCAAAAAAAAAA776040.2954406454740807No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAA759830.28926945215526356No Hit
ACGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA744020.2832505399794154No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAA687240.2616342317349714No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAA673300.2563272339025031No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAA647170.2463794682380558No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTCAAAAAAAAAA644670.2454277110944998No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAAAAAAAA595940.22687606085230613No Hit
TACGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAA588630.2240931229645484No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAA573950.21850440501758753No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCAAAAAAAAAAAA571370.21752219164543773No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAA570580.217221436388074No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCCGAAAAAAAAAA570160.21706154118795665No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGTAAAAAAAAA567540.21606409970150992No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAA557740.21233321169877037No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAA546260.20796274289556121No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAA519570.19780178363095732No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCTAAAAAAAA505910.1926013825985673No Hit
CCGCCTGGATACCGCAGCTAGGAATAACGAAAAAAAAAAAA491680.1871839809374465No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTTAAAAAAAAAA471140.1793643442459904No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAAA470880.17926536150306055No Hit
TCGACTGCATAATTTGTGGTAGTGGGAGAAAAAAAAAAAAA468670.17842400818815707No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAACGAAAAAAAAAA468020.1781765513308325No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGAAAAAAAAA456120.17364618732750592No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCACAAAAAAAAAA444730.16930998178146475No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAA438120.1667935358939027No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACGCAAAAAAA434240.16531640880710377No Hit
TCGACTGCATAATTTGTGGTAGTGGGCGAAAAAAAAAAAAA412270.15695236702953358No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGAAAAAAAA407100.15498413325665977No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGTAAAAAAAAAA407060.15496890514236286No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGTAAAAAAA405710.15445495628484263No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAA405340.15431409622759634No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA401180.15273037234071915No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGCAAAAAAAAAA400790.15258189822632443No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTAAAAAAA391530.14905658976659297No Hit
TCGCCGTCGGGTCCCGGGGGGTCGGTCACGCGTCAAAAAAA387040.14734723393676638No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAAAAAA369750.1407648815319331No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGGAAAAAAAAA367810.1400263179885336No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGTAAAAAAAAA363430.1383588394730235No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA358820.13660379930030622No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAA357750.13619644724286425No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAA355440.1353170236422185No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA349870.13319650872637573No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA348850.13280819181180487No Hit
TTACGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAA339010.12906207569476844No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA332890.12673217420734334No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA328120.12491622157743848No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAA326870.12444034300566048No Hit
TCGCCGTCGGGTCCCGGGGGGTCGGTCACGCGTAAAAAAAA325430.12389213089097223No Hit
TCGCCGGCGGGCCGCCGGTGAAATACCACTACTCTCAAAAA309190.11770951648643242No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCAAAAAA304080.11576412488500394No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA303260.11545194854191757No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGGAAAAAAAA301630.11483140288431905No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCCAAAAAAAAAAA297510.11326290711173875No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCAAAAAAAAAA292080.11119569059593512No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA291790.11108528676728262No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAA286310.10899903510860787No Hit
ACGCCGGCGGGCCGCCGGTGAAATACCACTACTCTCAAAAA284260.10821859425089195No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA282090.10739246905028532No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTTAAAAAAAAAA279050.10623513236372123No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGAAAAAA275350.10482653179125835No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAA271880.10350549287600261No Hit
CGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAAA265260.1009852399598663No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAA264600.10073397607396752No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA263000.10012485150209167No Hit

[OK]Adapter Content

Adapter graph