FastQCFastQC Report
Wed 20 Sept 2023
SRR18158440_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18158440_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18175826
Total Bases745.2 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA5098522.8051104802609794No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA4513552.4832709115943343No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAAA1684500.9267804390292909No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAAA1028820.5660375489950223No Hit
ACGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA874860.4813316324661119No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA841440.46294457264280586No Hit
AACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAA750820.4130871411291019No Hit
ACGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA744740.40974203868368897No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAAA702280.3863813396981243No Hit
TAGTCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAAAAA644560.3546248737196318No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAA591070.32519567473852357No Hit
TCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAAAAAAAA587340.3231434984027686No Hit
TCGACTGCATAATTTGTGGTAGTGGGCAAAAAAAAAAAAAA515430.283579959447235No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA484270.26643630941449375No Hit
TCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAAAA473110.26029628584692655No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAAAAAAA471830.2595920537531554No Hit
CTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAAAAAAAAA441720.24302609410983578No Hit
TATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAAA404220.2223942944876343No Hit
TTGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAAAAAAAAA403170.22181660409821266No Hit
AAATAGCTTAAGACACCTTGCCTAGCCACACAAAAAAAAAA399480.21978643501538803No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA373690.205597258688546No Hit
TCGACTGCATAATTTGTGGTAGTGGGTAAAAAAAAAAAAAA353960.1947421811806517No Hit
TCGACTGCATAATTTGTGGTAGTGGTGAAAAAAAAAAAAAA345550.190115156252046No Hit
CCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAAAAA345510.19009314899911564No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAAAA341250.18774937656203355No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTAAAAAAAAAAAAA339600.1868415773786567No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTAAAAAAAAAAAAAA336930.18537259324555594No Hit
TAGTCTAACGCGTGCGCGAGTCAGGGGAAAAAAAAAAAAAA330900.18205499986630594No Hit
ATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAAT328290.1806190266126007No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA327840.1803714450171343No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAA327330.18009085254227236No Hit
TTATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAA327020.17992029633206216No Hit
TCCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAAAA319110.17556836206508578No Hit
TGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA307310.16907622245063306No Hit
ACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAAAAAAAT298550.1642566340588868No Hit
TAGTTGGTGGAGCGATTTGTCTGGTTAAAAAAAAAAAAAAA290540.15984968165958455No Hit
AGGAGTCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAAA288100.1585072392308333No Hit
TGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAAAAAAAAAA279360.1536986544655522No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAA271560.1494072401441343No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA266210.1464637700647002No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTAAAAAAAAAAAAA265210.14591358874144153No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAAAA256850.14131407287899875No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAAA250770.1379689704335858No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAAAA244340.13443130452503232No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAA227720.12528729093247262No Hit
TGAGGTGGAGCACGAGCGTACGCGTTAGGAAAAAAAAAAAA227040.1249131676326567No Hit
TGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAA224840.12370276872148754No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAAAAAAAAA224650.12359823427006839No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA210270.11568662684160817No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAA207900.11438269710548506No Hit
GTCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAAAAAAA205610.11312278187522262No Hit
AGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAAAAAAAAAA202470.11139521252019027No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA198130.10900742557724749No Hit
TCGACTGCATAATTTGTGGTAGTAAAAAAAAAAAAAAAAAA197960.10891389475229353No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAAA196840.10829769167024375No Hit
ATGTACACGGAGTCGACCCAACGCGAACAAAAAAAAAAAAA195290.10744491061919277No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAA195130.10735688160747137No Hit
ATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAATG194310.10690573292239923No Hit
ACGGAGTCGACCCAACGCGAACAAAAAAAAAAAAAAAAAAA193980.10672417308572386No Hit
TCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAA188410.10365966311517286No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)