FastQCFastQC Report
Tue 19 Sept 2023
SRR18158441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18158441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27788354
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length41
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAA4696811.6902080634211007No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAA4027891.4494885159444852No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAA2406890.8661506183489674No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAA2044460.7357254769390084No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA1835970.6606976433364855No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA1643890.5915751613067833No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAA1527000.5495107770686958No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA1454350.5233667312572742No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAA1256190.45205628228285855No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAA1190000.4282369513501951No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA1130300.4067531311858198No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCAAAAAAAA996830.35872221866757564No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGTAAAAAAAA995580.35827238993716576No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA988960.35589009698091506No Hit
CCGCCTGGATACCGCAGCTAGGAATAACGAAAAAAAAAAAA973210.3502222549777507No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCCAAAAAAAAA954220.343388456905364No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGTAAAAAAAAA952980.34294222680479747No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGAAAAAAAAAAA939820.33820642993104233No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAA923180.33221830987182616No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA915740.3295409292684266No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA906940.32637413500634116No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAA858880.3090791199795425No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGAAAAAAAA839170.3019862205584397No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGAAAAAAA822500.2959873046096937No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAA814180.2929932445800856No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAAA794570.2859363314574156No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA789000.28393189463470925No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAA751290.2703614614957043No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA734840.2644417154035104No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAAA676050.24328537055487345No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTAAAAAAA663960.2389346270743492No Hit
GCCGCCTGGATACCGCAGCTAGGAATAACGAAAAAAAAAAA661790.2381537243983577No Hit
CCGCCTGGATACCGCAGCTAGGAATAAAAAAAAAAAAAAAA633720.22805237042827367No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCAAAAAAA610570.21972154234108288No Hit
TGAGAAGACGGTCGAACTTGACTATCTAGAAAAAAAAAAAA608310.21890825199650182No Hit
TTGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAAAAAAAAA596920.2148094126050071No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCTAAAAAAAA570050.2051398942161166No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAAAAAAAAAAAA566590.20389476829034206No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAAAAAAAAAAA565580.2035313066761709No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA543860.195715082656569No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAA532340.19156946107711165No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTCAAAAAAAAAA531470.1912563802807464No Hit
AAATAGCTTAAGACACCTTGCCTAGCCACACAAAAAAAAAA523270.18830550380925765No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTCAAAAAAAAA517490.18622549575984243No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAAAAAA515930.18566410950429088No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA514660.18520708351419446No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCCGAAAAAAAAA502530.1808419455142971No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAA496990.17884830458112058No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA494030.17778311014751No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCCGAAAAAAAAAA493570.17761757317471916No Hit
TTATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAA488500.17579306784417673No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGAAAAAAAAAA474100.17061104086985504No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGAAAAAAA473320.1703303477420793No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAA473270.1703123545928629No Hit
CTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAA465590.16754860687322465No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCAAAAAAAAAAAA460870.16585005358719698No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAA454630.16360450856499092No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGAAAAAAAAA450200.16201031554441836No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAA440680.1585844199336168No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA432340.15558316264432215No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAAA422420.15201332183978944No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCAAAAAAAAAAA409620.14740707564039238No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA407150.14651821406910248No Hit
TGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCAAAAAAAA400650.14417910467097117No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAA399200.1436573033436957No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGAAAAAAAA396180.14257051713102545No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAAA393750.14169605007910868No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAAA393280.14152691447647459No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA391970.141055493967005No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGAAAAAA386150.13896109139821666No Hit
GCCGCCTGGATACCGCAGCTAGGAATAAAAAAAAAAAAAAA382900.13779153669915103No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA379770.1366651655582047No Hit
TGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAA377250.1357583108376984No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAA377040.13568273961098953No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA374450.1347506944815803No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA374040.13460315065800588No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAA369300.13289740011229165No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAA357600.12868700319565526No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAAAA356140.12816160323853654No Hit
ACGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA355650.12798527037621588No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCCAAAAAAAAAA351410.1264594513226656No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAA349230.1256749500168308No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCAAAAAAAA348330.12535107333093568No Hit
TCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAAA336610.12113347915461276No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGAAAAAAAAAAAA328110.11807464378782566No Hit
TGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGAAAAAAA327830.11797388215221384No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAA323670.1164768521374098No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCAAAAAA320810.11544764400223202No Hit
CCGCCTGGATACCGCAGCTAGGAATAACAAAAAAAAAAAAA319810.11508778101790412No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAA315680.1136015468926299No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCAAAAAAA314280.11309773871457085No Hit
AACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAA314050.11301497022817544No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCAAAAAAAAAAAAA313690.11288541955381741No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAAAAAAAAA311210.11199295935268422No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGCAAAAAAAAAAA310850.11186340867832618No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCACAAAAAAAAAA308480.11101053340546907No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAAA308250.11092776491907363No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTAAAAAAAA307060.11049952796772346No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA302070.10870381167592727No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAA300310.10807045282351017No Hit
TGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAAA289410.1041479462943361No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGTAAAAAAAAA289190.10406877643778396No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAA287860.10359015866862786No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA287570.10348579840317278No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAAAAAA284410.10234863137269663No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCAAAAAAAAAAAA281980.10147416432077984No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGAAAAAA279390.10054211919137061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)