FastQCFastQC Report
Tue 19 Sept 2023
SRR18158442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18158442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20520907
Total Bases841.3 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA4511532.1985041889230335No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA2252041.0974368725514911No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA2130801.0383556633242381No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAAA1895350.9236190193737538No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAA1559110.7597666126550838No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA1332670.649420612841333No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA1270080.618920011673948No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAAA1215650.5923958429322836No Hit
AACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAA998920.48678160278198235No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAAA874320.4260630390264914No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA805830.39268732127678374No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAAAAAAA794190.38701505737538794No Hit
TTGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAAAAAAAAA778850.37953975426134917No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAA628820.3064289507281525No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAA628690.30636560070176233No Hit
ACGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA622790.30349048411943974No Hit
ACGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA559200.2725025750567458No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAA549640.2678439115775926No Hit
TAACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAA509730.2483954534758137No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTAAAAAAAAAAAAA473520.2307500345866779No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAA463380.2258087325282455No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAA433280.21114076487944708No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAAAAAAAAA429640.20936696414052264No Hit
AGGAGTCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAAA419900.20462058524021381No Hit
TTGGGGAAGGTGGCTCGGGGGAAAAAAAAAAAAAAAAAAAA412090.20081471057785114No Hit
TAGTCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAAAAA390100.1900988099600081No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAA389570.1898405367754944No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAA378130.18426573445316036No Hit
AAATAGCTTAAGACACCTTGCCTAGCCACACAAAAAAAAAA377460.1839392381633034No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAAAA363940.17735083541872684No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA346240.16872548567175905No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCAAAAAAAA333450.16249281769075802No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCACAAAAAAAAAA331640.1616107904002489No Hit
TGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAA329850.1607385092676459No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAA328710.16018297826699374No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAAA327750.1597151626874972No Hit
TCAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCAAAAAAAA323690.157736692632543No Hit
TCGACTGCATAATTTGTGGTAGTGGGCAAAAAAAAAAAAAA317940.15493467223451673No Hit
CGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAAA310080.15110443217738864No Hit
TGGAGTCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAAA308170.1501736740973486No Hit
TAGTCTAACGCGTGCGCGAGTCAGGGGAAAAAAAAAAAAAA307010.14960839693879027No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGAAAAAAAAAAAA303200.14775175385766331No Hit
TGAGGTGGAGCACGAGCGTACGCGTTAGGAAAAAAAAAAAA298800.1456075991183041No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAAA291840.14221593616695402No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCGAAAAAAAAAAA284840.13880478089979162No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTTAAAAAAAAAA281550.13720153792422526No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTAAAAAAAAAAAAAA278250.13559342186970585No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCTCAAAAAAAAAAAA277550.1352523063429896No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGAAAAAA274310.13367342876218874No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAAA269530.13134409702261213No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAA269520.13133922394365902No Hit
TCGACTGCATAATTTGTGGTAGTGGGTAAAAAAAAAAAAAA268720.13094937762741188No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTAAAAAAAAAAAAA264630.1289562883355984No Hit
TCGACTGCATAATTTGTGGTAGTGGGCGAAAAAAAAAAAAA263580.12844461504552407No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA255450.12448280185666258No Hit
CCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAAAAA254030.12379082464532391No Hit
TCGACTGCATAATTTGTGGTAGTGGAGGAAAAAAAAAAAAA250880.12225580477510084No Hit
TCGAATACAGACCGTGAAAGCGGGGCCCCAAAAAAAAAAAA249260.12146636598470038No Hit
TGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA245890.11982413837750934No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAA245770.11976566143007228No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTAAAAAAAAAAAAA243760.11878617256050135No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAAA241240.11755815666432289No Hit
TCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAAA241130.1175045527958389No Hit
TTGGGGAAGGTGGCTCGGGGGTAAAAAAAAAAAAAAAAAAA241020.11745094892735491No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAA240170.11703673721634233No Hit
TCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAAAAAAAA238040.11599877139933436No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA237150.11556506737250942No Hit
ACAACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAA236400.11519958645102772No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA236190.11509725179301286No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA229890.11202721205256667No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA229770.11196873510512961No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA228490.1113449809991342No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAA228020.11111594628833901No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA226800.11052143065606213No Hit
ACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAAAAAAAA226360.1103070151821262No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA223380.10885483765410563No Hit
TCGAATACAGACCGTGAAAGCGGGGCCACAAAAAAAAAAAA220100.1072564677574924No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAA216160.10533647464997527No Hit
CTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAA211890.10325566993700619No Hit
ACGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA211770.10319719298956913No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCAAAAAAAAAAA211400.10301688906830483No Hit
TTATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAA209240.10196430401443757No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCGAAAAAAAAAAA207680.10120410369775566No Hit
ACGATTAAAGTCCTACGTGATCTGAGCAAAAAAAAAAAAAA206210.10048776109165154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)