FastQCFastQC Report
Tue 19 Sept 2023
SRR18158443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18158443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25418348
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length35-81
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAAAA7805933.0709824257658287No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAAA1855530.7299963003103113No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAAAAAAAAAA1808820.7116198110121082No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAAAAAAAAAAAAAAA1735720.6828610576895083No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAAAAAAAAAAA1723260.6779590868769284No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCAAAAAAAAAAAAAAAA1490670.5864543203201089No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAAAA1212530.47702942771890605No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGTAAAAAAAAAAAAAAAAAA1211790.4767382994362969No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAAA1058700.4165101524300478No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAAA1033650.4066550666471322No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCCAAAAAAAAAAAAAAAAAA977250.3844663705131427No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGAAAAAAAAAAAAAAAAAAAA853930.33595023563293724No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAAA821000.32299502705683314No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCTAAAAAAAAAAAAAAAAA808900.31823468621957646No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAAAAAAAAAAA786650.30948116691139804No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA783240.30813961631180753No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGAAAAAAAAAAAAAAAAA775600.3051339135021678No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCAAAAAAAAAAAAAAAAA770560.3031510938476411No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGAAAAAAAAAAAAAAAAA724140.28488869536289296No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCAAAAAAAAAAAAAAAA709040.27894810473127524No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA641180.25225085438282613No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGAAAAAAAAAAAAAAAAAAA627200.2467508903411032No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAAA618320.2432573509497942No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGAAAAAAAAAAAAAAAA602010.2368407262344508No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAAAAAAAAAAAA601930.23680925290660115No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAAAAAAAAAAAA589490.23191515042598362No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAAAAAAAAAAA584490.2299480674353817No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCAAAAAAAAAAAAAAAAA583610.22960186082903578No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGAAAAAAAAAAAAAAAA582990.22935794253820113No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAAAAAAA579250.2278865644612309No Hit
CAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGAAAAAAAAAAAAAAAAA574930.22618700475735085No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGAAAAAAAAAAAAAAAAA551050.21679221639423615No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGAAAAAAAAAAAAAAAA468560.1843392812152859No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAAA425430.16737122333835386No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAAA424560.1670289508979891No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCCGAAAAAAAAAAAAAAAAAA414330.16300429909921763No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAAAT411230.16178470764504443No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAAAAAAAT399870.1573154950903969No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAAAAAAAAAAAAAAAAAA387290.1523663142860425No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA379450.1492819281567787No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAAAAAAAAAAAAAAAAAAAA371800.14627229118115778No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTCAAAAAAAAAAAAAAAAAAA366830.1443170106884995No Hit
TACGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAAAAAAAAAA364630.14345149417263467No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCCGAAAAAAAAAAAAAAAAAAA361960.14240107185565326No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAAAAAAAAAAAAAAAAAA355210.13974550981834066No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCCAAAAAAAAAAAAAAAAAAA353590.13910817492938565No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAATG350540.1379082543051185No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAAAAAAAAAAA348570.13713322360682134No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAAA339760.13366722337738077No Hit
CAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCAAAAAAAAAAAAAAAAAA337190.1326561427202114No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA326390.12840724346051127No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGAAAAAAAAAAAAAAA317770.1250159923847136No Hit
TCCCTACCTACTATCCAGCGAAACCACAGCCAAAAAAAAAAAAAAAAAAA313820.12346199682213808No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAAAA312980.12313152687971696No Hit
TGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGAAAAAAAAAAAAA312670.12300956773429964No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTCAAAAAAAAAAAAAAAAAA303260.11930751754598687No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAAAAAAAAAAA300380.11817447774340017No Hit
GCCGCCTGGATACCGCAGCTAGGAATAACGAAAAAAAAAAAAAAAAAAAA295950.11643164221372689No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAAAT292030.11488944914909499No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAAAAAA279350.10990092668492854No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAAAAAAAAAAAA275190.10826431363674775No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAAAAAAAAAAAA273550.10761911041583033No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAAAAAA266800.10496354837851775No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAAAAAAAAAAAAAAAAAAAAT264860.1042003201781642No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAAAAAAAAAAAAAAAA262870.10341742114790467No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAAAAAAAAAATGG261210.10276434959502483No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAAAA258840.10183195225747953No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)