FastQCFastQC Report
Tue 19 Sept 2023
SRR18158444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18158444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16589401
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length35-81
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAATG1326500.7996069297499048No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAATGG721980.4352055869889455No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAAAAAAAAATGG569510.3432975066429463No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAAAAAAAAAAA564420.340229282540099No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAAAAAAAAA551130.3322181433796193No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA475950.2869000514244004No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAAAAAAAATGG458130.2761582530918386No Hit
AAATAGCTTAAGACACCTTGCCTAGCCACACAAAAAAAAAAAAAAAAAAA449460.27093202461017124No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAAA439600.2649884706506281No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAAA368480.2221177244434564No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAAAAAAAAAA366730.2210628340348154No Hit
AACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAAAAAAAAATG354340.21359420994163683No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAAAT349080.2104235107705215No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAAAAAAAAAAAAAAAAAA336590.20289460722542063No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAAA329090.19837364833124477No Hit
TTATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAAAAAAAATGG303010.1826527672698972No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAATGGA283470.170874162364271No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAAAAAAAAAAA280660.1691803097652531No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAAAAAAAATGGA265910.16028909060670726No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAAAA251740.15174749226931097No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTAAAAAAAAAAAAAAAAAAA248500.14979443802702702No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA246280.14845623419435094No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAAAAAAT243140.14656345940398935No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAAAAAAAAAAT228330.13763607257429006No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAAAAAAAAAATG221700.1336395449118386No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAAAAAAATGGA219420.1322651734080091No Hit
ACGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAATG216710.13063160026091358No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA211450.12746090108979824No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAAAAAAAAAAA208950.12595391479173962No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA198820.11984760631200608No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAAA198730.11979335480527596No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAAAAAAAAAAAAA192590.116092196457244No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAAAAAAAAAATG187790.11319878276497145No Hit
TATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAAAAAAAATGGA177850.10720700524389035No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAAAAAAAAT175840.10599538826025123No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTAAAAAAAAAAAAAAAAAAAATG175040.10551315264487246No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAAAAAAAAATG173950.1048561066189189No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAAAAAAAAAAAAAAAT172190.10379518826508563No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATTAAAAAAAAAAAAAAAAAAAA170060.1025112359391397No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTTAAAAAAAAAAAAAAAAAAA168170.10137195429780738No Hit
TTATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAAAAAAATGGA167160.1007631318333917No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCACAAAAAAAAAAAAAAAAAAA166790.10054009786127903No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAAAAAAAAAATG166240.10020856087570612No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAAAA165950.10003375046513131No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)