FastQCFastQC Report
Tue 19 Sept 2023
SRR18158445_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18158445_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28641870
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length41
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA3288431.1481198678717557No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAA2257130.7880525957278627No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA2088110.729041085655371No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAA1582570.5525372470442746No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAA1385040.4835717779600284No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA1356450.4735898878110961No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAA1345780.46986457239000107No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA1333130.4654479613237544No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAA1186060.41410005701443375No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAAAAAAAAAAA1140820.3983049989403625No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA1109150.38724776001008315No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAA1070530.3737640035374785No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAA1070100.37361387367514765No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA1070030.373589433930117No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTCAAAAAAAAA1064540.3716726596412874No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA1016720.35497682239323064No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAA1008090.35196375097017063No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAAA1000900.3494534400163118No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTAAAAAAAA999290.3488913258806077No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAAA998590.3486469284303015No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGTAAAAAAAA977430.34125914264676155No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAA906340.31643883587209914No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAA886260.3094281204404601No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCCGAAAAAAAAA885810.3092710077938347No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTAAAAAAAAA859920.3002317935246546No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAAA774660.27046418407736644No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAAAAAAA751220.26228036088425793No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAA731870.2555245170793667No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAA729650.25474942802268147No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAA728750.2544352027294307No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCCAAAAAAAAAA690830.24119584370713223No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTAAAAAAA671350.23439461180432705No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAA655950.22901786789759188No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA652010.22764225939158303No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA650110.22697889488360923No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTCAAAAAAAAAA628780.21953175543356634No Hit
GCCGCCTGGATACCGCAGCTAGGAATAACGAAAAAAAAAAA620440.216619934382776No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA613410.21416548570327287No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACGCAAAAAAA589810.20592580023580864No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAA580410.20264389161741186No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGTAAAAAAAAA542550.18942548094799677No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA542510.18941151537940784No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCACAAAAAAAAAA539980.1885281931661585No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGAAAAAAA536240.18722241250309424No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCAAAAAA515480.1799742824054435No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGTAAAAAAAAA514120.17949945307342013No Hit
TCGACTGCATAATTTGTGGTAGTGGGAGAAAAAAAAAAAAA511980.17875229515391278No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCAAAAAAAAAAAA505120.17635720014091258No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAA504770.1762350014157595No Hit
ACGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA480590.16779281520375589No Hit
CCGCCTGGATACCGCAGCTAGGAATAACGAAAAAAAAAAAA471670.1646784934084262No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCAAAAAAA465530.1625347786300266No Hit
TTATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAA463360.16177714653407754No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTTAAAAAAAAAA462430.1614524470643851No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAA437810.15285663959790335No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCCGAAAAAAAAAA433550.15136930654318312No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAA432290.15092939113263204No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAAAAAAAA426830.14902309102024414No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAA418580.1461426924987789No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA415210.14496609334516217No Hit
TCGACTGCATAATTTGTGGTAGTGGGCGAAAAAAAAAAAAA414580.14474613563988664No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAAA414300.14464837665976418No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCAAAAAAAA402930.14067866378836297No Hit
ACGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA401160.14006068737830316No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCGTAAAAAAAAAA396080.13828706016751002No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGAAAAAAA392960.13719774581757405No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGATAAAAAAAAAAA379820.13261005653611305No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAAA378440.1321282444197952No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGAAAAAA376520.13145789712752695No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAA363710.12698542378692454No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAAAAAA360130.12573550539821596No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGAAAAAAAAAAA359180.12540382314422904No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGAAAAAAAA359140.12538985757564014No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGAAAAAAAAA349350.12197178466350138No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA334320.11672422226621376No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACAAAAAAAAAA333030.11627383267922102No Hit
TCGCCGTCGGGTCCCGGGGGGTCGGTCACGCGTAAAAAAAA329540.11505533681983754No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAA326800.11409869537149635No Hit
ACGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA324870.11342485668708084No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAAAAA322580.11262532788536503No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCGAAAAAAAAAAA321160.11212955020045828No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA320320.11183627326009091No Hit
TACGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAA318000.1110262702819334No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAAAAAA316290.11042924222475697No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAA309440.108037638603904No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAACGAAAAAAAAAA308150.10758724901691126No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA307760.10745108472316926No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGTAAAAAAAAAA307750.10744759333102204No Hit
TGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGAAAAAAA304370.10626750278525808No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAAA303150.10584155294329596No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAA300620.10495823073004659No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAAAAAA300620.10495823073004659No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCAAAAAAAAAA295590.10320206047998962No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACTAAAAAA294010.10265042052072716No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA293100.10233270383532918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)