FastQCFastQC Report
Tue 19 Sept 2023
SRR18158446_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18158446_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22464532
Total Bases921 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA2952081.3141070555131085No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA2143200.954037235229294No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA1605780.71480678965402No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA1574690.7009671957555136No Hit
TTATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAA1429840.6364877754853739No Hit
TATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAAA1251390.5570514444725578No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAAA1233660.5491590031788777No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAAAA1179260.5249430524526396No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAA919650.40937865965781084No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAAA900470.40084075644219963No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAAA899810.4005469599811828No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA894580.39821884560070064No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA735550.3274272528802291No Hit
AACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAA708890.31555965643976025No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAA623700.2776376556609325No Hit
ACGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA531520.23660408327224447No Hit
ACGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA504930.22476764706248942No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAA503180.22398864129464172No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAA502610.22373490798739984No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAAA491150.21863353307337985No Hit
TCGACTGCATAATTTGTGGTAGTGGGCAAAAAAAAAAAAAA484120.21550415561739722No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAA481230.21421768323506585No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAA480850.21404852769690463No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA480720.21399065869700737No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAAAAAAA469090.20881360893696782No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAA450940.20073420625900418No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA438190.19505859280754212No Hit
ACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCAAAAAAAA434710.19350948419490777No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTAAAAAAAAAAAAA430350.19156864696758427No Hit
CTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAA426780.18997947520117492No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAA424100.18878648351098523No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA403070.17942505991222074No Hit
TTGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAAAAAAAAA400270.17817865068366437No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAAA392940.174915729381765No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAA389780.17350906753810852No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAA383490.17070909823538724No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAA381390.16977429131396993No Hit
CTCAGGATGGCGGAGCGGTCTCACTGAACGTCAAAAAAAAA380540.16939591708387247No Hit
TCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAAAA373800.16639563201227606No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAA371640.16543411632167543No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAA370990.16514477132218913No Hit
TAGTCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAAAAA368950.1642366731699552No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAA357840.15929109940950473No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA350060.15582786233873022No Hit
ACGGAGTCGACCCAACGCGAACAAAAAAAAAAAAAAAAAAA349590.15561864364679398No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA349180.15543613372404108No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA333200.14832269819820862No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCACAAAAAAAAAA331850.14772175089158324No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTAAAAAAAAAAAAA331550.14758820704566647No Hit
CGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAAA330400.14707628896965225No Hit
TCAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCAAAAAAAA328940.1464263755861907No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA324190.14431193135917544No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCGAAAAAAAAAAA320740.14277617713113275No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAA320270.1425669584391965No Hit
AGGAGTCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAAA310110.13804427352414908No Hit
TGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAA305030.1357829310666254No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCTCAAAAAAAAAAAA299670.13339694768624602No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAA298880.13304528222533193No Hit
AAATAGCTTAAGACACCTTGCCTAGCCACACAAAAAAAAAA295870.1317053923046338No Hit
TCGAATACAGACCGTGAAAGCGGGGCCCCAAAAAAAAAAAA294110.1309219350752555No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAA291610.12980906969261588No Hit
ACGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA287780.128104159926412No Hit
TCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAAA283970.12640815308326922No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAAA282020.1255401180848103No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA281730.12541102570042412No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAA278180.12383075685707585No Hit
TCGAATACAGACCGTGAAAGCGGGGCCACAAAAAAAAAAAA276570.12311407155065593No Hit
TCGACTGCATAATTTGTGGTAGTGGGTAAAAAAAAAAAAAA272470.12128897232312695No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA271920.12104414193894625No Hit
AACGCGCCCGATCTCGTCTGATCTCGGAAGCAAAAAAAAAA271290.12076369986252106No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGAAAAAA271240.12074144255486827No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCAAAAAAAAAAAA267130.11891189186580874No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTAAAAAAAAAAAA266640.11869377025081136No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAA265490.11818185217479715No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCAAAAAAAAAAAAA262490.1168464137156296No Hit
ACGATTAAAGTCCTACGTGATCTGAGCAAAAAAAAAAAAAA258100.11489222210371443No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCGAAAAAAAAAAA257730.11472751802708377No Hit
TGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAAA246740.10983536180499999No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA244290.10874475373001316No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAAAAAAAAA240190.10691965450248418No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAAA239240.10649676565708112No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA238580.10620296919606426No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAA238260.10606052242708638No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAA237030.1055129926588277No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCAAAAAA235590.10487198219842728No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA230620.10265960581773972No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAA228890.1018895029729531No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAA228150.10156009481969176No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAAAA225710.1004739382062355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)