FastQCFastQC Report
Tue 19 Sept 2023
SRR18158447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18158447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28078106
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length41
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA3755661.3375759746757847No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA3328551.1854610136452937No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA2568850.9148943308355627No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA2452610.8734955270843411No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA2013860.7172349872886725No Hit
TTATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAA1845720.6573520307958094No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAA1815440.646567827616293No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA1794730.6391919739885589No Hit
TATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAAA1673970.5961833750467357No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAAA1642700.585046583982552No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAA1585290.5646000481656419No Hit
ATGTACACGGAGTCGACCCAACGCGAACAAAAAAAAAAAAA1470500.5237176610131752No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAAAA1265600.4507426533684288No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA1168410.4161284952767113No Hit
TGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA1099230.39149008127542506No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAA1043930.3717950206470479No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA1043800.3717487212278492No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA1038270.3697792151650115No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA1022840.3642838302555023No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA932170.3319917661112897No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAA828770.29516592037938744No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAAA785900.2798977965251645No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAA776360.2765001314547356No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA772970.27529278506178445No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAA758990.27031381675103017No Hit
AAATAGCTTAAGACACCTTGCCTAGCCACACAAAAAAAAAA744310.2650855438753597No Hit
ACGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA743570.26482199333530543No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA741600.26412037905975566No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA675900.24072136489548118No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAA661100.23545035409439655No Hit
TTGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAAAAAAAAA628320.22377577746875094No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAA613550.21851545114901982No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA597570.21282418408136217No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA577750.20576530340045016No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCGAAAAAAAAAAA572080.2037459364246292No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA568560.20249229061247934No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAAA560680.19968583351028021No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAA555390.19780180329827088No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAA537080.1912807081788209No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAA524660.18685733289845122No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAA522940.18624475596751433No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAAA508300.18103072906698192No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA501150.17848426101105253No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAAA501150.17848426101105253No Hit
AACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAAAA498150.17741581287569752No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAA489530.17434580523344417No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTAAAAAAAAAAAA487630.173669121414386No Hit
ATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAAAAA484150.1724297215773742No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTAAAAAAAAAAAAA483540.1722124704565187No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAA482530.1718527595842825No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAA473550.16865453816578654No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAA472380.1682378433929981No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAAAA471650.16797785434672838No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAA451000.16062336968170146No Hit
AGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA444690.15837606710367144No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCTCAAAAAAAAAAAA436730.1555411180511962No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAA432630.15408090559954435No Hit
ACGTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAA429930.15311930227772486No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAA425940.15169826625770272No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTAAAAAAAAAAA424060.15102870542621358No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAA422440.15045174343312187No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAA411650.1466088916396284No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAA401280.14291562258508464No Hit
ACGGAGTCGACCCAACGCGAACAAAAAAAAAAAAAAAAAAA396970.14138061876395794No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAA385630.13734188481231605No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCAAAAAAAAAAAAA379340.13510170522185505No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAAA375930.13388723584133488No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAA373300.132950562976007No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAAAAAAAAAA367900.131027356332368No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGAAAAAAAAAAAA367510.13088845807477184No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAAAAAAAAAAAA366390.13048957077090598No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAAAA365100.13003013807270336No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAA364450.12979864097670976No Hit
ACGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA358840.12780064296359592No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAA345070.12289646602231646No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA340830.12138639265768139No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCGAAAAAAAAAAA338660.12061354850644128No Hit
TCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAAAA333760.11886841655202812No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAAAAAAA333690.11884348609553651No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCACAAAAAAAAAA333020.1186048660119739No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCGAAAAAAAAAAAAA327790.11674220476267168No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCAAAAAAAAAAA325060.11576991695949862No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA324370.11552417388836697No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAA320250.1140568384491461No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCGAAAAAAAAAAAA305650.10885705752375179No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAA299910.10681276009143921No Hit
ACGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA299070.10651359461353982No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCAAAAAAAAAAAA297750.10604347743398362No Hit
ACGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA297280.10587608722611133No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA296670.10565883610525581No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAA288610.1027882721149354No Hit
TGGGCCGCCGGTGAAATACCACTACTCCCAAAAAAAAAAAA282510.10061576090638022No Hit
TGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAAAAAAAAAA281270.10017413567710017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)