FastQCFastQC Report
Tue 19 Sept 2023
SRR18158448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18158448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23814836
Total Bases976.4 Mbp
Sequences flagged as poor quality0
Sequence length41
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAA6572632.7598888356820934No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAA4168561.750404663714669No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA4047191.6994406344011774No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAA3914281.6436308862257123No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCAAAAAAA2846651.1953263083566898No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAAA2726941.14505932352421No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAAAAAA1621810.6810082588853436No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAA1594430.6695112240117883No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAA1585030.6655641046614807No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA1440700.604959026381706No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAAA1432430.6014864011660631No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAA1419270.5959604340756325No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAAAAAAAAAAA1321620.5549565825269592No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAA1265150.5312444729831438No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCCGAAAAAAAAA1183170.49682055337269593No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAAAA1178110.49469582742455165No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAA1145640.48106146941343625No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTCAAAAAAAAA1122980.471546392341312No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGAAAAAAAAAAA1061800.4458565240592041No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCCAAAAAAAAAA998720.41936883378075757No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCAAAAAAAAAAAA939570.3945313753157906No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTCAAAAAAAAAA895180.3758917340434341No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCCGAAAAAAAAAA889800.37363263807485386No Hit
TCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAAA866480.3638404228355803No Hit
TGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAAAAAAAAAA815270.3423370205026816No Hit
TTGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAAAAAAAAA779860.3274681379288104No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAA762260.3200777868048304No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGTAAAAAAAAA714490.3000188621916187No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAA695360.2919860544074291No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAAA695120.2918852768921021No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAAA681890.2863299163597012No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAA662040.2779947760295305No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAAAAAAAAAAAA658950.27669726551969537No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCCAAAAAAAAAAA630920.26492729154212946No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGAAAAAAAA624920.2624078536589544No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTAAAAAAAA597310.25081424033321076No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGAAAAAAAAAA596690.25055389841861603No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCAAAAAAAAAAAA580030.24355825922966673No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA574660.24130336232422508No Hit
GCCGCCTGGATACCGCAGCTAGGAATAAAAAAAAAAAAAAA561420.2357438027286856No Hit
GCCGCCTGGATACCGCAGCTAGGAAAAAAAAAAAAAAAAAA549570.23076791290941495No Hit
TCCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAAAA531190.2230500348606222No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAA529740.22244117070552155No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCAAAAAAAAAAAAA526850.22122764145845894No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA514610.2160879881767819No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCAAAAAA510660.21442935823702503No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAA506600.21272453860274326No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAA501360.21052422951810373No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGAAAAAAAAAAAAA491520.20639235138969675No Hit
TTGGTGACTCTAGATAACCTCGGGCCAAAAAAAAAAAAAAA486090.20411226010542335No Hit
CGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAAAA477250.20040028829087886No Hit
AGGAGTCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAAA467930.19648676144568034No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA450320.18909221125856168No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAAA449560.18877308246002616No Hit
TCGACTGCATAATTTGTGGTAGTGGGCAAAAAAAAAAAAAA449090.1885757264925108No Hit
TGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAAAAAAAAAT440340.1849015462462139No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAA438690.1842087008283408No Hit
ACGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAA436790.18341087883200202No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAAAA434390.18240310367873205No Hit
ACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAAAAA426820.1792244128827929No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA419650.17621368461239875No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAAA413480.17362286265586713No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGTAAAAAAAAA406480.17068351845882962No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA406360.17063312970116612No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAA404850.16999907116723373No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAAAAAAAA398470.16732006888479098No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGAAAAAAAAAAAA393510.16523733356803297No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAA378340.15886735478673883No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAA368300.1546514953955593No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAA359200.15083034793941055No Hit
ACTGACCCGGTGAGGCGGGGGAAAAAAAAAAAAAAAAAAAA357160.14997373905913106No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGTAAAAAAA347610.14596363376174415No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAA344390.14461153543110689No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA342050.14362895465666864No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTAAAAAAAAA339410.14252040198807164No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCAAAAAAAAAAAA338630.1421928750632589No Hit
TCGACTGCATAATTTGTGGTAGTGGGAGAAAAAAAAAAAAA323990.13604544662831186No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCAAAAAAAAAAA310600.130422901085693No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAA309980.1301625591710982No Hit
GCCGCCTGGATACCGCAGCTAGGAATAACGAAAAAAAAAAA307330.1290498074393626No Hit
TTGGTGACTCTAGATAACCTCGGGCAAAAAAAAAAAAAAAA304540.1278782688236862No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA303350.12737858031018984No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGGAAAAAAAAA303310.12736178405763535No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAAAAA296420.1244686295551227No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA293270.12314592466645582No Hit
TCCCTACCTACTATCCAGCGAAACCACAGCCAAAAAAAAAA291460.1223858942383647No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAA286720.12039553831065643No Hit
ACGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA281980.11840518238294818No Hit
CCGCCTGGATACCGCAGCTAGGAATAAAAAAAAAAAAAAAA280730.11788029949062004No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCTAAAAAAAA278920.11712026906252893No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA278420.11691031590559767No Hit
ACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAAAAAAAA275460.11566739321656468No Hit
TTATAACCTAGACTTACAAGTCAAAGTAAAAAAAAAAAAAA269140.11301358531295366No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAA268420.11271125276697265No Hit
GCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAA265110.11132136286808778No Hit
GCCGCCTGGATACCGCAGCTAGGAACAAAAAAAAAAAAAAA256610.10775215920025652No Hit
CCGCCTGGATACCGCAGCTAGGAAAAAAAAAAAAAAAAAAA254010.10666040278421401No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAAAAAAAAAAA253540.10646304681669863No Hit
TCGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAAAA252960.10621950115465838No Hit
TGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAA246140.10335574009411612No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAAAAA242820.10196165113209263No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTTAAAAAAAAAA240440.10096227410509988No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAAA240160.10084470033721836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)