FastQCFastQC Report
Fri 29 Sept 2023
SRR18220721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18220721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87855426
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC28661433.2623403362701806Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCT13607441.5488445756326992TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT9706391.1048139474049103No Hit
AAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGTCTGAAC8954591.0192415434875928Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG8215670.9351351844791009TruSeq Adapter, Index 12 (100% over 49bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACG7659100.8717845156200141No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA5810630.6613854447646751Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTCTAGCATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGA5226570.5949057716708357Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGCCACCTTACAAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTGA5102720.5808087482268881Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTC4789190.5451217093865096TruSeq Adapter, Index 12 (98% over 50bp)
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA4734580.5389058155611243Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTC4299740.4894108646175138No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC3803820.43296358269323054No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC3488460.3970682470995019No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC3385350.38533192019352336No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA3298560.3754531905633239Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3050660.3472363790029315No Hit
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT2892690.32925570243094604TruSeq Adapter, Index 12 (100% over 35bp)
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA2793280.31794052196616746Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT2615630.29771980162044853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2502250.2848145087817342TruSeq Adapter, Index 12 (100% over 50bp)
GTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC2259940.2572339698176411Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTC2244070.25542759305498103TruSeq Adapter, Index 12 (100% over 50bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG2218580.25252623554520126Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2158510.24568886616063984Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA2150370.24476234399000013No Hit
GTGACTGGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2088250.23769163671234148TruSeq Adapter, Index 12 (100% over 43bp)
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCAC2033090.23141314003758856No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG1966810.22386892757198626No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1861700.2119049539410349No Hit
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1786390.2033329165121799Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGTCTGA1748250.19899169346694645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1743450.1984453413270115Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCT1698860.19336995759374043TruSeq Adapter, Index 12 (100% over 50bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCAC1691510.19253335587946496No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG1649780.18778350696290516TruSeq Adapter, Index 12 (100% over 50bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC1593620.1813911869256658No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGT1592360.18124776948893287No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCTG1589720.18094727581196862Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1565300.17816770930004938Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC1559380.17749387499412958No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACG1519250.1729261434575481No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1507790.1716217277234533No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTAGATCGGAAGAGCACACGTCTGA1504290.17122334595475072Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1470760.16740684861057983Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1460590.1662492650140926No Hit
AACGCCGCCGCATCGCCGGTCGGCATCGTTTAGATCGGAAGAGCACACGT1456760.16581332153576947No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTC1363200.15516400774153663No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1343620.15293534630405184No Hit
ATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1341600.1527054231118292Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA1302650.14827200314298175Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1269670.14451810864817843TruSeq Adapter, Index 12 (100% over 50bp)
ACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTCTGAACT1262880.1437452480168954Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTTCCGCCTGGGCGGGATTCTGACTTAGATCGGAAGAGCACACGTCT1255450.14289954043362102Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1178060.1340907504107942No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1146520.13050076155797138No Hit
CGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAACT1138550.12959358935895432Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGC1107320.1260388857485023TruSeq Adapter, Index 12 (100% over 50bp)
GAATACAAGCTTGGGCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1084840.12348013655980679Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC1070800.12188205655049694No Hit
AAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT1069580.12174319204826348TruSeq Adapter, Index 12 (100% over 35bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA1045870.1190444401237096No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGT1034750.11777872433286021TruSeq Adapter, Index 12 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT1031170.11737123669515871TruSeq Adapter, Index 12 (100% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC1005760.11447898505437787TruSeq Adapter, Index 12 (100% over 50bp)
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT987470.11239715575450059TruSeq Adapter, Index 12 (100% over 35bp)
GCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACA977420.1112532309615117No Hit
CGACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAAC969290.1103278470244968Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA955380.1087445640523102Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT953650.10854764963520866TruSeq Adapter, Index 12 (100% over 36bp)
AAAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACG941740.10719201338799496No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG929560.10580564483290993No Hit
TAGAGCCACCTTACAAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTG928550.1056906832367986Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA920730.10480058454215452No Hit
CGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA916120.10427585884109196Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGCAATTTTTGACAGTCTCTACGGAGACTGAGATCGGAAGAGCACACGT911310.1037283684675321No Hit
AATTTTTGACAGTCTCTACGGAGACTGAGATCGGAAGAGCACACGTCTGA901200.10257761427279403Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAAGCATCTGGCCTACACCCAGAAGATTAGATCGGAAGAGCACACGTC898050.1022190706809617No Hit
GTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTCTG886090.10085774326562369Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)