FastQCFastQC Report
Sat 30 Sept 2023
SRR18220722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18220722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99838495
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC21591822.1626748279809305Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA19192731.9223777361627898Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGTCTGAAC13961311.398389468911766Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT13623231.3645267789743825No Hit
GAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCT7930690.7943519180652713TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG7856580.7869289295676982TruSeq Adapter, Index 4 (100% over 49bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG6870440.688155405387471TruSeq Adapter, Index 4 (100% over 35bp)
GGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTC5434890.5443681818320679TruSeq Adapter, Index 4 (98% over 50bp)
GAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCACACGTCTGAACTCCA5369660.5378346298188891Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT4773480.47812018800964495Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC4360770.4367824254562331Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGAACGTGCGGTGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3985890.39923378252045966Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGACTGGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC3951310.39577018864316815TruSeq Adapter, Index 4 (100% over 43bp)
ACGAAACCCCGACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2584420.25886007195921773Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAGAGATCCTTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2549580.25537043602269843Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGCGCGCGCGCGCGCGCGGACAGATCGGAAGAGCACACGTCTGAACTCC2398800.24026804490592532Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACGCCGCCGCATCGCCGGTCGGCATCGTTTAGATCGGAAGAGCACACGT2163040.21665390689232644No Hit
AAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA2073640.20769944498862888TruSeq Adapter, Index 4 (100% over 36bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC2018720.20219856078559678No Hit
TGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAA1960890.19640620584274632Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTTCCGCCTGGGCGGGATTCTGACTTAGATCGGAAGAGCACACGTCT1940720.19438594301727005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGAGGCCAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG1923360.19264713475498604TruSeq Adapter, Index 4 (100% over 35bp)
ATGAACGCTTGGCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1888520.18915749881846675Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCACACGTCTGAACTCC1819460.1822403272405098Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGT1814270.1817204876736173No Hit
AATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1809470.1812397111955664Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1781050.17839311379844017Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGT1773050.17759181966835538No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1720020.1722802412035558No Hit
AGGTCGACCCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC1711160.1713928079544869TruSeq Adapter, Index 4 (100% over 37bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG1710150.1712916445705637No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1680270.16829881099469698TruSeq Adapter, Index 4 (100% over 50bp)
ATGGTGACCTCCCGGGAGCGGGGGACCACCAGGAGATCGGAAGAGCACAC1650210.16528794830090338No Hit
AGCGCCAGGTTCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1558210.15607306580492825Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTC1546160.15486611652148802TruSeq Adapter, Index 4 (100% over 50bp)
AAGCTTGGGCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA1537220.15397067033111828TruSeq Adapter, Index 4 (100% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480830.1483225483316831No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCAC1472730.14751123802497224No Hit
AAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG1464200.14665685815876933TruSeq Adapter, Index 4 (100% over 35bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCT1446270.1448609576897168TruSeq Adapter, Index 4 (100% over 50bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC1395900.13981580952317038No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1332390.13345453574795973Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTCCGCCTGGGCGGGATTCTGACTTAGATCGGAAGAGCACACGTCTGAA1302520.13046270378975564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCACGCGCGCGCGCGCGCGCGGACAGATCGGAAGAGCACACGTCTGAA1286170.12882505891139484Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCCCGGCTCCACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1273980.12760408698067816Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGTACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAAC1256640.12586728195371935Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCCGGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1226480.12284640308329968Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGACTGGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG1215530.12174963174274613TruSeq Adapter, Index 4 (97% over 45bp)
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA1204680.12066287657881863No Hit
TAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACG1178610.1180516593324048No Hit
AAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGTCTGAA1178590.1180496560970796Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG1155160.11570286591359374TruSeq Adapter, Index 4 (100% over 35bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCTGA1126680.11285025881049189Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCACACGTCTGAACTCC1120690.11225028983059089Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG1095860.10976327317434023TruSeq Adapter, Index 4 (100% over 50bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1064660.10663822606700951Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1059390.10611037355881617Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1033210.10348813851811368Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCCGGGCCCGCGCCCTAGGCTTCAGATCGGAAGAGCACACGTCTGAACT1032380.10340500425211738Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTCTGA1026130.10277899321298864Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)